概述
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产品名称Anti-Anillin抗体
参阅全部 Anillin 一抗 -
描述山羊多克隆抗体to Anillin
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宿主Goat
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经测试应用适用于: WB, ELISAmore details
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种属反应性与反应: Mouse, Human
预测可用于: Rat, Dog
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免疫原
Synthetic peptide:
C-TYPDDEKRKNP
, corresponding to internal sequence amino acids 1018-1028 of Human Anillin -
阳性对照
- Human or mouse Brain lysate.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab52059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| ELISA |
应用说明
Peptide ELISA: Antibody detection limit dilution 1:16,000.
WB: Use at a concentration of 1.0 - 3.0 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 124 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1.0 - 3.0 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 124 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.
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组织特异性Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
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序列相似性Contains 1 PH domain.
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发展阶段Expressed in fetal brain, heart, kidney, liver, lung, skeletal muscle, spleen and thymus. In dividing cells expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter G1 phase.
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翻译后修饰Phosphorylated during mitosis.
Ubiquitinated, and this requires FZR1/CDH1. -
细胞定位Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
- Information by UniProt
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数据库链接
- Entrez Gene: 54443 Human
- Entrez Gene: 68743 Mouse
- Entrez Gene: 363031 Rat
- Omim: 616027 Human
- SwissProt: Q9NQW6 Human
- SwissProt: Q8K298 Mouse
- Unigene: 62180 Human
- Unigene: 282751 Mouse
see all -
别名
- Actin binding protein scraps homolog Drosophila antibody
- Actin binding protein anillin antibody
- Actin-binding protein anillin antibody
see all
图片
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Western blot - Anillin antibody (ab52059)Anti-Anillin antibody (ab52059) at 1 µg/ml + Mouse Brain lysate (35µg protein in RIPA buffer).
Predicted band size: 124 kDa
Observed band size: 100 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
文献
ab52059 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"