Anti-ANGPTL2抗体(ab36014)

概述

  • 产品名称Anti-ANGPTL2抗体
    参阅全部 ANGPTL2 一抗
  • 描述
    兔多克隆抗体to ANGPTL2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from within residues 1 - 100 of Human ANGPTL2.

    (Peptide available as ab36013.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysate: Tagged ANGPTL2 recombinant protein.

性能

应用

Our Abpromise guarantee covers the use of ab36014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 80 (size of the recombinant protein + tag) kDa (predicted molecular weight: 57 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Induces sprouting in endothelial cells through an autocrine and paracrine action.
    • 组织特异性Widely expressed in heart, small intestine, spleen and stomach. Also found in lower levels in colon, ovary, adrenal gland, skeletal muscle and in prostate.
    • 序列相似性Contains 1 fibrinogen C-terminal domain.
    • 翻译后修饰N-glycosylated.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AI593246 antibody
      • Angiopoietin like 2 antibody
      • Angiopoietin related protein 2 antibody
      • Angiopoietin-like protein 2 antibody
      • Angiopoietin-related protein 2 antibody
      • ANGL2_HUMAN antibody
      • Angptl2 antibody
      • Arp2 antibody
      • AW260363 antibody
      • HARP antibody
      • MGC8889 antibody
      • UNQ170/PRO196 antibody
      see all

    Anti-ANGPTL2 antibody 图像

    • Anti-ANGPTL2 antibody (ab36014) at 1 µg/ml + ANGPTL2 Recombinant Protein at 0.1 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 57 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    Anti-ANGPTL2 antibody (ab36014)参考文献

    ab36014 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.


    The antibody has not been tested against other proteins, to our knowledge. I aligned the immunogen sequence (*********) with mouse ANGPTL3 and ANGPTL4 and there was no significant homology.


    H...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Cell lysate - whole cell (Rat Cardiomyoctes)
    Loading amount 20 µg
    Specification Rat Cardiomyoctes
    Gel Running Conditions Reduced Denaturing (8% SDS-Page gel with a 5% stacking gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Prof. Paul Townsend

    Verified customer

    提交于 Mar 24 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Intestine, Kidney)
    Loading amount 20 µg
    Specification Intestine, Kidney
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 12 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (kidney)
    Specification kidney
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 05 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"