Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human ANGPTL2.
(Peptide available as
This antibody gave a positive signal in the following lysate:
Tagged ANGPTL2 recombinant protein.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 79 (size of the recombinant protein + tag) kDa (predicted molecular weight: 57 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Induces sprouting in endothelial cells through an autocrine and paracrine action.
Widely expressed in heart, small intestine, spleen and stomach. Also found in lower levels in colon, ovary, adrenal gland, skeletal muscle and in prostate.
Contains 1 fibrinogen C-terminal domain.
Information by UniProt
Angiopoietin like 2 antibody
Angiopoietin related protein 2 antibody
Western blot - ANGPTL2 antibody (ab35574)
Anti-ANGPTL2 antibody (ab35574) at 1 µg/ml + ANGPTL2 Recombinant Protein at 0.1 µg
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size:
Observed band size:
80 kDa (
why is the actual band size different from the predicted?
ab35574 was tested on a recombinant tagged ANGPTL2 protein with a predicted molecular weight of 79.6 kDa.
This product has been referenced in:
et al. Angiopoietin-like Protein 2 Is a Multistep Regulator of Inflammatory Neovascularization in a Murine Model of Age-related Macular Degeneration. J Biol Chem 291:7373-85 (2016).
Read more (PubMed: 26839315) »
et al. Bioinformatics analysis identifies miR-221 as a core regulator in hepatocellular carcinoma and its silencing suppresses tumor properties. Oncol Rep 32:1200-10 (2014).
Read more (PubMed: 24993451) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"