概述

  • 产品名称Anti-ANGPT1 + ANGPT2抗体
  • 描述
    兔多克隆抗体to ANGPT1 + ANGPT2
  • 经测试应用适用于: ICC/IF, IHC-Fr, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to a sequence mapping near the C-terminus of human Angiopoietin 2, identical to the related rat and mouse sequence. (Peptide available as ab98299.)

  • 阳性对照
    • Human placenta tissue, rat brain lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab65835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 20562294
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 21439064

Anti-ANGPT1 + ANGPT2 antibody 图像

  • This image shows human placental tissue stained with ab65835 at 2µg/ml.
  • Anti-ANGPT1 + ANGPT2 antibody (ab65835) at 2 µg/ml + rat brain tissue lysate

    Predicted band size : 57 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab65835 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65835, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.



  • Predicted band size : 57 kDa

Anti-ANGPT1 + ANGPT2 antibody (ab65835)参考文献

This product has been referenced in:
  • Chaitanya GV  et al. Inflammation induces neuro-lymphatic protein expression in multiple sclerosis brain neurovasculature. J Neuroinflammation 10:125 (2013). WB, IHC-P ; Mouse, Human . Read more (PubMed: 24124909) »
  • Milton I  et al. Age-dependent lethality in novel transgenic mouse models of central nervous system arteriovenous malformations. Stroke 43:1432-5 (2012). IHC-Fr ; Mouse . Read more (PubMed: 22328553) »

See all 10 Publications for this product

Product Wall

Thank you for contacting us.

The online datasheet is the best source for information about this antibody, ab65385:

Click here (or use the following: http://www.abcam.com/index.html?datasheet=65385).

There are six refere...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

Read More

Thank you for your reply.
I have aligned the human angiopoietin 1 and 2 proteins and the overall sequence homology is only 61%.
I would recommend ab64015, since the immunogen does not align well with Ang1 (63% homology)
http://www.abcam....

Read More

Thank you for contacting us.
I am very sorry to hear that you had not been informed about the recall for ab65835. I believe it was sent to the person placing the order with us.
I would be more than happy to send you a replacement antibody for...

Read More

Thank you for your participation in Abcam's phone survey.

If there is anything else I can do for you, or if you have questions to any of our products (e.g. primary antibodies, lysates, secondary antibodies, kits, controls, gels, proteins/pept...

Read More

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.
To check the status of the order please contact our Customer Service team and r...

Read More

Thank you for contacting Abcam.
For ab15429 we have found that there are problems with this antibody in western blot and that we can no longer guarantee that it will work in this application. However it is still guaranteed to work in IP and IHC-P....

Read More

Thank you for your reply. I very much appreciate your honest feedback as well as for sharing you findings with the knockout data. I will definitely pass the WB data on to the supplying lab - for their consideration and re-evalution of the antibody. ...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I will pass them on to the lab for further evaluation. I am sorry this product did not perform as s...

Read More

Thank you for contacting us. Your credit note ID is XXXXXXX. I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the...

Read More

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"