Anti-Angiopoietin 1 + Angiopoietin 2抗体(ab65835)
Key features and details
- Rabbit polyclonal to Angiopoietin 1 + Angiopoietin 2
- Suitable for: ICC/IF, WB
- Reacts with: Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Angiopoietin 1 + Angiopoietin 2抗体 -
描述
兔多克隆抗体to Angiopoietin 1 + Angiopoietin 2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Rat -
免疫原
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阳性对照
- WB: Rat brain tissue lysate. ICC: PC12 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate, 2.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65835于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa).
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说明 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa). |
靶标
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数据库链接
- Entrez Gene: 89805 Rat
- SwissProt: 89807 Rat
- SwissProt: O35460 Rat
- SwissProt: O35462 Rat
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别名
- AGP1 antibody
- AGPT antibody
- AGPT2 antibody
see all
图片
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Anti-Angiopoietin 1 + Angiopoietin 2 antibody (ab65835) at 2 µg/ml + rat brain tissue lysate
Predicted band size: 57 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted? -
ICC/IF image of ab65835 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65835, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab65835 被引用在 15 文献中.
- Klomjit N et al. Microvascular remodeling and altered angiogenic signaling in human kidneys distal to occlusive atherosclerotic renal artery stenosis. Nephrol Dial Transplant 37:1844-1856 (2022). PubMed: 35451482
- Lun W et al. MiR-218 regulates epithelial-mesenchymal transition and angiogenesis in colorectal cancer via targeting CTGF. Cancer Cell Int 18:83 (2018). PubMed: 29977158
- Prapansilp P et al. A clinicopathological correlation of the expression of the angiopoietin-Tie-2 receptor pathway in the brain of adults with Plasmodium falciparum malaria. Malar J 12:50 (2013). IHC-P ; Human . PubMed: 23383853
- Chaitanya GV et al. Inflammation induces neuro-lymphatic protein expression in multiple sclerosis brain neurovasculature. J Neuroinflammation 10:125 (2013). WB, IHC-P ; Mouse, Human . PubMed: 24124909
- Milton I et al. Age-dependent lethality in novel transgenic mouse models of central nervous system arteriovenous malformations. Stroke 43:1432-5 (2012). IHC-Fr ; Mouse . PubMed: 22328553