RabMAb

Anti-Angiotensin II Type 2 Receptor抗体[EPR3876] (ab92445)

概述

  • 产品名称Anti-Angiotensin II Type 2 Receptor抗体[EPR3876]
    参阅全部 Angiotensin II Type 2 Receptor 一抗
  • 描述
    兔单克隆抗体[EPR3876] to Angiotensin II Type 2 Receptor
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues at the C terminal of Human Angiotensin II Type 2 Receptor.

  • 阳性对照
    • HepG2, 293T, HeLa and fetal liver lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab92445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
IP 1/10 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IHC-P.
  • 靶标

    Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] 图像

    • All lanes : Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 41 kDa

    Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445)参考文献

    This product has been referenced in:
    • Alhusban A  et al. Clinically relevant doses of candesartan inhibit growth of prostate tumor xenografts in vivo through modulation of tumor angiogenesis. J Pharmacol Exp Ther 350:635-45 (2014). Read more (PubMed: 24990940) »
    • Alhusban A  et al. AT1 receptor antagonism is proangiogenic in the brain: BDNF a novel mediator. J Pharmacol Exp Ther 344:348-59 (2013). Rat . Read more (PubMed: 23211364) »

    See all 3 Publications for this product

    Product Wall


    AT2R is a 41 kDa protein that contains a large number of potential glycosylation sites, and depending on the amount of post-translational glycosylation, the protein may run at a variety of sizes between 41 and 58 kDa. The SwissProt link below depi...

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    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forWB a...

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    According to our records, 92445 Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] was proving difficult to use inWB and we were in contact in order to help resolve the issue.

    Looking at our correspondence, it appears that we are awaitin...

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    Yes of course, thanks for your reply.

    We don't have any specific recommendations for western blotting protocol for ab32212. Although a good starting dilution for western blotting using ab32212 is noted as 1:5000.

    ab52174 is antibody...

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    Thanks for your email.

    I'm sorry but I don't quite understand your question. What is it that you requested for ab32213 and ab52174?

    Thanks for the additional details.

    Thank you for your enquiry.

    i have checked and none ofthese 3 antibodies have a specific western blot protocol associated with them, although typically a 1:1000 dilution, or 1ug/ml primaryantibody concentration works well as a starting point ...

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    Thanks for your email.

    For ab11734 I'd recommend starting with a dilution of 1: 250 and dilute ab87436 to 1:1000. The primary antibody diluent could be TBS with 0.05% Tween 20.

    I hope this is helpful. Please contact me again if you ...

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    Thank you for your enquiry.

    Here is an antibody that has be used in western blotting and IHC on rat samples for ACE 1:
    http://www.abcam.com/Angiotensin-Converting-Enzyme-1-antibody-9B9-ab77990.html

    Here is an antibody that has ...

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    Thanks for your enquiry. For western blotting, its recommended to use 1ug/ml as the starting concentration for ab2742. This may need to be optimized. For the antibody diluent, you can use TBS/0.5% Tween 20 (TBST). I hope this is helpful. Please let me ...

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    Glad to hear your experiments are working well. I would be happy to help you troubleshoot your experiments with ab11734. So I can help you in the most efficient manner, please provide some more details regarding the application, sample and sample prepa...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"