Anti-Angiotensin II Type 2 Receptor抗体(ab19134)

概述

  • 产品名称Anti-Angiotensin II Type 2 Receptor抗体
    参阅全部 Angiotensin II Type 2 Receptor 一抗
  • 描述
    兔多克隆抗体to Angiotensin II Type 2 Receptor
  • 特异性We have received mixed feedback regarding the use of this antibody in western blot. There are abReviews and specific references that reported the successful use of this antibody in western blot, but the lab's testing found this antibody to be unsuitable for western blot; thus, we have removed western blot as a guaranteed application. We welcome any additional feedback from researchers who have used this antibody in western blot.
  • 经测试应用适用于: ICC/IF, Electron Microscopy, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Sheep, Rabbit
  • 免疫原

    Synthetic peptide:

    CRKSSSLREMETFVS

    conjugated to KLH, corresponding to amino acids 349-363 of Rat Angiotensin II Type 2 Receptor.

性能

应用

Our Abpromise guarantee covers the use of ab19134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
Electron Microscopy Use at an assay dependent concentration. PubMed: 19539732
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

Anti-Angiotensin II Type 2 Receptor antibody 图像

  • ab19134 at 1/2000 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum prior to incubation with the antibody for 18 hours. An HRP conjugated horse polyclonal antibody was used as the secondary.

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  • ICC/IF image of ab19134 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Angiotensin II Type 2 Receptor antibody (ab19134)参考文献

This product has been referenced in:
  • Wang J  et al. Qishenyiqi Dropping Pill attenuates myocardial fibrosis in rats by inhibiting RAAS-mediated arachidonic acid inflammation. J Ethnopharmacol 176:375-84 (2015). WB ; Rat . Read more (PubMed: 26590099) »
  • Goyal R  et al. Antenatal maternal protein deprivation: sexually dimorphic programming of the pancreatic renin-angiotensin system. J Renin Angiotensin Aldosterone Syst 14:137-45 (2013). Read more (PubMed: 22898440) »

See all 13 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Bos taurus Tissue sections (ovary follicle)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate and citric acid buffer
Permeabilization No
Specification ovary follicle
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 17 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (Aorta)
Specification Aorta
Permeabilization No
Fixative Formalin 10%
Username

Abcam user community

Verified customer

提交于 Jul 21 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (epididymis)
Specification epididymis
Fixative bouin's solution
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 12 2013

Thank you for contacting us.

HEK293 lysate, Hela cell lysate, and human testis lysate have all been shown to have good results in WB for Angiotensin II type 2 Receptor. We do have these lysates available in our catalog as ab7902, ab150035, and...

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Thank you for contacting us.
The credit note was issued and its ID is xxxxx.
I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be u...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Brain)
Loading amount 30 µg
Specification Brain
Treatment Diluted 1:2 in sample buffer treated with ß-mercaptoethanol
Gel Running Conditions Reduced Non-Denaturing (Native) (4-20% Tris-HCl pre-cast gels)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Miss. Laura Palmer

Verified customer

提交于 Nov 19 2012

Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital informa...

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Thank you for your enquiry. I can confirm the following concentrations: ab18801 Angiotensin II Type 1 Receptor   2.1 mg/ml ab19134 Angiotensin II Type 2 Receptor 1.6 mg/ml as stated on the datasheet I hope this will be helpful to yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"