Anti-Angiotensin II Type 1 Receptor抗体(ab124505)

概述

  • 产品名称
    Anti-Angiotensin II Type 1 Receptor抗体
    参阅全部 Angiotensin II Type 1 Receptor 一抗
  • 描述
    兔多克隆抗体to Angiotensin II Type 1 Receptor
  • 特异性
    ab124505 is specific for Angiotensin II Type 1 Receptor (AGTR1) and will not cross-react with AGTR2.
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Angiotensin II Type 1 Receptor (internal sequence).
    Database link: P30556
    (Peptide available as ab176148)

  • 阳性对照
    • Mouse kidney tissue lysate; Mouse kidney tissue, mouse kidney cells.

性能

应用

Our Abpromise guarantee covers the use of ab124505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 41 kDa.Can be blocked with Angiotensin II Type 1 Receptor peptide (ab176148).
IHC-P Use a concentration of 2.5 µg/ml.

靶标

  • 功能
    Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • 组织特异性
    Liver, lung, adrenal and adrenocortical adenomas.
  • 疾病相关
    Defects in AGTR1 are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 翻译后修饰
    C-terminal Ser or Thr residues may be phosphorylated.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AG2S antibody
    • Agtr 1 antibody
    • Agtr1 antibody
    • AGTR1_HUMAN antibody
    • Agtr1a antibody
    • AGTR1B antibody
    • Ang II antibody
    • Angiotensin II receptor type 1 antibody
    • Angiotensin II type-1 receptor antibody
    • Angiotensin receptor 1 antibody
    • Angiotensin receptor 1B antibody
    • AT 1B antibody
    • AT 1r antibody
    • AT1 antibody
    • At1a antibody
    • AT1AR antibody
    • AT1B antibody
    • AT1BR antibody
    • AT1R antibody
    • AT2R1 antibody
    • AT2R1A antibody
    • AT2R1B antibody
    • HAT1R antibody
    • Type 1 angiotensin II receptor antibody
    • Type 1B angiotensin II receptor antibody
    • Type-1 angiotensin II receptor antibody
    see all

图片

  • Lane 1 : Anti-Angiotensin II Type 1 Receptor antibody (ab124505) at 1 µg/ml
    Lane 2 : Anti-Angiotensin II Type 1 Receptor antibody (ab124505) at 2 µg/ml

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse kidney tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 41 kDa
  • Immunofluorescence of AGTR1 in mouse kidney cells using ab124505 at 20 ug/ml.

  • Immunohistochemical analysis of mouse kidney tissue section labelling Angiotensin II Type 1 Receptor with ab124505, at 2.5 µg/ml.

  • ab124505, at 2.5 µg/ml, staining Angiotensin II Type 1 Receptor in paraffin-embedded Mouse kidney tissue by Immunohistochemistry.

文献

This product has been referenced in:
  • Czikora I  et al. Caveolin-1 prevents sustained angiotensin II-induced resistance artery constriction and obesity-induced high blood pressure. Am J Physiol Heart Circ Physiol 308:H376-85 (2015). Read more (PubMed: 25527780) »

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Sample
Human Tissue lysate - whole (Human Skeletal Muscle)
Specification
Human Skeletal Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Oct 17 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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