The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. In a simple indirect ELISA, using 1.0 ug of Angiotensin I coated per well. 50% binding was observed at an antibody dilution of 1/12K. Under the same conditions, the end point titre was observed at an antibody dilution of 1/100K.