Anti-Angiotensin Converting Enzyme 2抗体(ab87436)


  • 产品名称Anti-Angiotensin Converting Enzyme 2抗体
    参阅全部 Angiotensin Converting Enzyme 2 一抗
  • 描述
    兔多克隆抗体to Angiotensin Converting Enzyme 2
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Hamster, Cat, Dog, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Angiotensin Converting Enzyme 2.

    (Peptide available as ab87437.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysate: Human Kidney This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7.



Our Abpromise guarantee covers the use of ab87436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.


  • 功能Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
  • 组织特异性Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
  • 序列相似性Belongs to the peptidase M2 family.
  • 翻译后修饰N-glycosylation on Asn-90 may limit SARS infectivity.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACE 2 antibody
    • ACE related carboxypeptidase antibody
    • ACE-related carboxypeptidase antibody
    • ACE2 antibody
    • ACE2_HUMAN antibody
    • ACEH antibody
    • Angiotensin converting enzyme 2 antibody
    • Angiotensin converting enzyme homolog antibody
    • Angiotensin converting enzyme like protein antibody
    • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
    • Angiotensin I converting enzyme 2 antibody
    • Angiotensin-converting enzyme homolog antibody
    • DKFZP434A014 antibody
    • EC 3.4.17 antibody
    • metalloprotease MPROT 15 antibody
    • Metalloprotease MPROT15 antibody
    • OTTHUMP00000022963 antibody
    • Processed angiotensin-converting enzyme 2 antibody
    see all

Anti-Angiotensin Converting Enzyme 2 antibody 图像

  • ICC/IF image of ab87436 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87436 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Angiotensin Converting Enzyme 2 antibody (ab87436) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 92 kDa
    Observed band size : 92 kDa
    Additional bands at : 115 kDa (possible glycosylated form).

    Exposure time : 30 seconds
    ACE 2 contains multiple glycosylation sites (SwissProt) which may account for the characteristic reaction observed around the predicted molecular weight.

Anti-Angiotensin Converting Enzyme 2 antibody (ab87436)参考文献

This product has been referenced in:
  • Gao H  et al. Maternal protein restriction reduces expression of angiotensin I-converting enzyme 2 in rat placental labyrinth zone in late pregnancy. Biol Reprod 86:31 (2012). WB, IHC-P ; Rat . Read more (PubMed: 22011389) »
  • Gao H  et al. Protein restriction to pregnant rats increases the plasma levels of angiotensin II and expression of angiotensin II receptors in uterine arteries. Biol Reprod 86:68 (2012). WB ; Rat . Read more (PubMed: 22088913) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Heart)
Specification Heart
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Mar 25 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (cardiomyocytes)
Loading amount 25 µg
Specification cardiomyocytes
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Prof. Paul Townsend

Verified customer

提交于 Mar 24 2010

Application Western blot
Sample Mouse Tissue lysate - whole (Intestine, Kidney)
Loading amount 20 µg
Specification Intestine, Kidney
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Shunyou Wang

Verified customer

提交于 Mar 03 2010