Anti-Angiotensin Converting Enzyme 2抗体(ab15348)

概述

  • 产品名称Anti-Angiotensin Converting Enzyme 2抗体
    参阅全部 Angiotensin Converting Enzyme 2 一抗
  • 描述
    兔多克隆抗体to Angiotensin Converting Enzyme 2
  • 经测试应用适用于: IHC-Fr, ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Cat, Human, Ferret, Macaque Monkey
  • 免疫原

    Synthetic peptide:

    CKGENNPGFQNTDDVQTSF

    , corresponding to C terminal amino acids 788-805 of Human ACE2. (Peptide available as ab15352.)

  • 阳性对照
    • Human kidney or human testis cell lysate
  • 常规说明


    No cross reactivity to ACE1.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab15348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa).Can be blocked with Human Angiotensin Converting Enzyme 2 peptide (ab15352).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
  • 组织特异性Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
  • 序列相似性Belongs to the peptidase M2 family.
  • 翻译后修饰N-glycosylation on Asn-90 may limit SARS infectivity.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACE 2 antibody
    • ACE related carboxypeptidase antibody
    • ACE-related carboxypeptidase antibody
    • ACE2 antibody
    • ACE2_HUMAN antibody
    • ACEH antibody
    • Angiotensin converting enzyme 2 antibody
    • Angiotensin converting enzyme homolog antibody
    • Angiotensin converting enzyme like protein antibody
    • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
    • Angiotensin I converting enzyme 2 antibody
    • Angiotensin-converting enzyme homolog antibody
    • DKFZP434A014 antibody
    • EC 3.4.17 antibody
    • metalloprotease MPROT 15 antibody
    • Metalloprotease MPROT15 antibody
    • OTTHUMP00000022963 antibody
    • Processed angiotensin-converting enzyme 2 antibody
    see all

Anti-Angiotensin Converting Enzyme 2 antibody 图像



  • Predicted band size : 97 kDa

    Western blot analysis of ACE2 in human kidney lysate with ab15438 at 0.5  μg/mL (lane A), 1  μg/mL (lane B), and 2  μg/mL (lane C).

  • ab15348 at 2µg/ml staining Angiotensin Converting Enzyme 2 in human kidney by IHC

  • ICC/IF image of ab15348 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15348, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab15348 staining angiotensin converting enzyme 2 in human thoracic aortic sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized and blocked with a proprietary kit. Samples were incubated with primary antibody (2 µg/ml in TBST + Sodium azide) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

Anti-Angiotensin Converting Enzyme 2 antibody (ab15348)参考文献

This product has been referenced in:
  • Thatcher SE  et al. Angiotensin-Converting Enzyme 2 Decreases Formation and Severity of Angiotensin II-Induced Abdominal Aortic Aneurysms. Arterioscler Thromb Vasc Biol 34:2617-23 (2014). Read more (PubMed: 25301841) »
  • Honorato-Sampaio K  et al. Evidence that angiotensin-(1-7) is an intermediate of gonadotrophin-induced oocyte maturation in the rat preovulatory follicle. Exp Physiol 97:642-50 (2012). IHC-P ; Rat . Read more (PubMed: 22247282) »

See all 6 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (Reduced Non-Denaturing (Native) (4-20% Tris-HCl pre-cast gels))
Sample Human Tissue lysate - whole (Brain)
Specification Brain
Treatment Diluted 1:2 in sample buffer treated with Þ-mercaptoethanol
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Miss. Laura Palmer

Verified customer

提交于 Sep 06 2013

Hello and thank you for contacting Abcam. Although these products should be suitable to work together with ab15352 blocking ab15248, we do not have the WB image on file. Please let me know if I can be of further assistance.

Thank you for contacting us. We do not offer conjugation services, unfortunately, but rather offer the conjugation kits (www.abcam.com/EasyLink). However, we do not carry Alexa Fluor conjugation kits or Alexa Fluor conjugated secondary antibodies. ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (human thoraci aortic section- fresh frozen)
Specification human thoraci aortic section- fresh frozen
Fixative Acetone
Permeabilization Yes - NOVACASTRA KIT COMPONENT
Blocking step (agent) for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 16 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Ferret Tissue sections (respiratory tract)
Specification respiratory tract
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid buffer pH 6.0
Permeabilization No
Username

Abcam user community

Verified customer

提交于 Sep 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cat Tissue sections (respiratory tract)
Specification respiratory tract
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid buffer pH 6.0
Permeabilization No
Username

Abcam user community

Verified customer

提交于 Sep 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1%
Username

Miss. Laura Palmer

Verified customer

提交于 May 22 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"