Anti-Angiotensin Converting Enzyme 2抗体(ab15348)

概述

  • 产品名称
    Anti-Angiotensin Converting Enzyme 2抗体
    参阅全部 Angiotensin Converting Enzyme 2 一抗
  • 描述
    兔多克隆抗体to Angiotensin Converting Enzyme 2
  • 经测试应用
    适用于: IHC-Fr, ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Cat, Human, Ferret, Macaque monkey
  • 免疫原

    Synthetic peptide:

    CKGENNPGFQNTDDVQTSF

    , corresponding to C terminal amino acids 788-805 of Human ACE2. (Peptide available as ab15352.)

  • 阳性对照
    • Human kidney or human testis cell lysate
  • 常规说明


    No cross reactivity to ACE1.

性能

应用

Our Abpromise guarantee covers the use of ab15348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa).Can be blocked with Human Angiotensin Converting Enzyme 2 peptide (ab15352).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
  • 组织特异性
    Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
  • 序列相似性
    Belongs to the peptidase M2 family.
  • 翻译后修饰
    N-glycosylation on Asn-90 may limit SARS infectivity.
  • 细胞定位
    Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACE 2 antibody
    • ACE related carboxypeptidase antibody
    • ACE-related carboxypeptidase antibody
    • ACE2 antibody
    • ACE2_HUMAN antibody
    • ACEH antibody
    • Angiotensin converting enzyme 2 antibody
    • Angiotensin converting enzyme homolog antibody
    • Angiotensin converting enzyme like protein antibody
    • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
    • Angiotensin I converting enzyme 2 antibody
    • Angiotensin-converting enzyme homolog antibody
    • DKFZP434A014 antibody
    • EC 3.4.17 antibody
    • metalloprotease MPROT 15 antibody
    • Metalloprotease MPROT15 antibody
    • OTTHUMP00000022963 antibody
    • Processed angiotensin-converting enzyme 2 antibody
    see all

图片



  • Predicted band size : 97 kDa

    Western blot analysis of ACE2 in human kidney lysate with ab15438 at 0.5  μg/mL (lane A), 1  μg/mL (lane B), and 2  μg/mL (lane C).

  • ab15348 at 2µg/ml staining Angiotensin Converting Enzyme 2 in human kidney by IHC

  • ICC/IF image of ab15348 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15348, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab15348 staining angiotensin converting enzyme 2 in human thoracic aortic sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized and blocked with a proprietary kit. Samples were incubated with primary antibody (2 µg/ml in TBST + Sodium azide) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Delforce SJ  et al. Expression of renin-angiotensin system (RAS) components in endometrial cancer. Endocr Connect 6:9-19 (2017). Read more (PubMed: 27956412) »
  • Leoncikas V  et al. Generation of 2,000 breast cancer metabolic landscapes reveals a poor prognosis group with active serotonin production. Sci Rep 6:19771 (2016). Read more (PubMed: 26813959) »

See all 14 Publications for this product

客户评价及客户问答

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (Reduced Non-Denaturing (Native) (4-20% Tris-HCl pre-cast gels))
Sample
Human Tissue lysate - whole (Brain)
Specification
Brain
Treatment
Diluted 1:2 in sample buffer treated with Þ-mercaptoethanol
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Miss. Laura Palmer

Verified customer

提交于 Sep 06 2013

Hello and thank you for contacting Abcam. Although these products should be suitable to work together with ab15352 blocking ab15248, we do not have the WB image on file. Please let me know if I can be of further assistance.

Thank you for contacting us. We do not offer conjugation services, unfortunately, but rather offer the conjugation kits (www.abcam.com/EasyLink). However, we do not carry Alexa Fluor conjugation kits or Alexa Fluor conjugated secondary antibodies. ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (human thoraci aortic section- fresh frozen)
Specification
human thoraci aortic section- fresh frozen
Fixative
Acetone
Permeabilization
Yes - NOVACASTRA KIT COMPONENT
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 16 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Ferret Tissue sections (respiratory tract)
Specification
respiratory tract
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid buffer pH 6.0
Permeabilization
No
Username

Abcam user community

Verified customer

提交于 Sep 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (respiratory tract)
Specification
respiratory tract
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid buffer pH 6.0
Permeabilization
No
Username

Abcam user community

Verified customer

提交于 Sep 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1%
Username

Miss. Laura Palmer

Verified customer

提交于 May 22 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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