Anti-Angiotensin Converting Enzyme 1抗体[EPR2757] (ab75762)


  • 产品名称Anti-Angiotensin Converting Enzyme 1抗体[EPR2757]
    参阅全部 Angiotensin Converting Enzyme 1 一抗
  • 描述
    兔单克隆抗体[EPR2757] to Angiotensin Converting Enzyme 1
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
    不适用于: ICC or IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    within Human Angiotensin Converting Enzyme 1 aa 1250 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P12821

  • 阳性对照
    • Fetal kidney, fetal heart and fetal lung lysates; human kidney and spleen tissues.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Our Abpromise guarantee covers the use of ab75762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 195 kDa (predicted molecular weight: 150 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for ICC or IP.
  • 靶标

    • 功能Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
    • 组织特异性Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
    • 疾病相关Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
      Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
      Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
      Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    • 序列相似性Belongs to the peptidase M2 family.
    • 翻译后修饰Phosphorylated by CK2 on Ser-1299; which allows membrane retention.
    • 细胞定位Secreted and Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ACE 1 antibody
      • ACE antibody
      • ACE T antibody
      • ACE_HUMAN antibody
      • ACE1 antibody
      • Angiotensin converting enzyme somatic isoform antibody
      • Angiotensin converting enzyme testis specific isoform antibody
      • Angiotensin I converting enzyme 1 antibody
      • Angiotensin I converting enzyme antibody
      • Angiotensin I converting enzyme peptidyl dipeptidase A 1 antibody
      • angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript antibody
      • Angiotensin-converting enzyme antibody
      • Carboxycathepsin antibody
      • CD 143 antibody
      • CD143 antibody
      • CD143 antigen antibody
      • DCP 1 antibody
      • DCP antibody
      • DCP1 antibody
      • Dipeptidyl carboxypeptidase 1 antibody
      • Dipeptidyl carboxypeptidase I antibody
      • Kininase II antibody
      • MGC26566 antibody
      • MVCD3 antibody
      • Peptidase P antibody
      • Peptidyl dipeptidase A antibody
      • soluble form antibody
      • Testicular ECA antibody
      see all

    Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] 图像

    • All lanes : Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762) at 1/1000 dilution

      Lane 1 : fetal kidney lysate
      Lane 2 : fetal heart lysate
      Lane 3 : fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 150 kDa
      Observed band size : 195 kDa (why is the actual band size different from the predicted?)
    • ab75762 at 1/100 dilution staining Angiotensin Converting Enzyme 1 in human kidney by Immunohistochemistry, Paraffin-embedded tissue.
    • ab75762 at 1/100 dilution staining Angiotensin Converting Enzyme 1 in human spleen by Immunohistochemistry, Paraffin-embedded tissue. Note positive staining of endothelial cells.

    Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)参考文献

    This product has been referenced in:
    • Yang J  et al. Pathological Ace2-to-Ace enzyme switch in the stressed heart is transcriptionally controlled by the endothelial Brg1-FoxM1 complex. Proc Natl Acad Sci U S A 113:E5628-35 (2016). Read more (PubMed: 27601681) »
    • Zhou L  et al. Multiple Genes of the Renin-Angiotensin System Are Novel Targets of Wnt/ß-Catenin Signaling. J Am Soc Nephrol N/A:N/A (2014). Read more (PubMed: 25012166) »

    See all 3 Publications for this product

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    Thank you for contacting us. The immunogen of ab75762 shares 57% identity with the testis-specific isoform of Angiotensin Converting Enzyme 1. Thus, I would not predict that it would recognize this isoform. I hope this information is helpful to ...

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