Anti-Angiotensin Converting Enzyme 1抗体(ab85955)

概述

  • 产品名称Anti-Angiotensin Converting Enzyme 1抗体
    参阅全部 Angiotensin Converting Enzyme 1 一抗
  • 描述
    兔多克隆抗体to Angiotensin Converting Enzyme 1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1250 to the C-terminus of Human Angiotensin Converting Enzyme 1.

    (Peptide available as ab95056.)

  • 阳性对照
    • This antibody gave a positive signal in human lung and kidney tissue lysates.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab85955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 150 kDa).

靶标

  • 功能Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
  • 组织特异性Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
  • 疾病相关Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
    Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
  • 序列相似性Belongs to the peptidase M2 family.
  • 翻译后修饰Phosphorylated by CK2 on Ser-1299; which allows membrane retention.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACE 1 antibody
    • ACE antibody
    • ACE T antibody
    • ACE_HUMAN antibody
    • ACE1 antibody
    • Angiotensin converting enzyme somatic isoform antibody
    • Angiotensin converting enzyme testis specific isoform antibody
    • Angiotensin I converting enzyme 1 antibody
    • Angiotensin I converting enzyme antibody
    • Angiotensin I converting enzyme peptidyl dipeptidase A 1 antibody
    • angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript antibody
    • Angiotensin-converting enzyme antibody
    • Carboxycathepsin antibody
    • CD 143 antibody
    • CD143 antibody
    • CD143 antigen antibody
    • DCP 1 antibody
    • DCP antibody
    • DCP1 antibody
    • Dipeptidyl carboxypeptidase 1 antibody
    • Dipeptidyl carboxypeptidase I antibody
    • Kininase II antibody
    • MGC26566 antibody
    • MVCD3 antibody
    • Peptidase P antibody
    • Peptidyl dipeptidase A antibody
    • soluble form antibody
    • Testicular ECA antibody
    see all

Anti-Angiotensin Converting Enzyme 1 antibody 图像

  • All lanes : Anti-Angiotensin Converting Enzyme 1 antibody (ab85955) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 150 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • ICC/IF image of ab85955 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85955, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Angiotensin Converting Enzyme 1 antibody (ab85955)参考文献

This product has been referenced in:
  • Casselbrant A  et al. The muscular expression of RAS in patients with achalasia. J Renin Angiotensin Aldosterone Syst N/A:N/A (2014). Human . Read more (PubMed: 24468662) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Primary lung endothelial cell)
Permeabilization Yes - Acetone
Specification Primary lung endothelial cell
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde
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Ms. Luana dos Santos Ortolan

Verified customer

提交于 Jan 05 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"