Anti-Angiotensin Converting Enzyme 1抗体[2E2] (ab11734)

概述

  • 产品名称Anti-Angiotensin Converting Enzyme 1抗体[2E2]
    参阅全部 Angiotensin Converting Enzyme 1 一抗
  • 描述
    小鼠单克隆抗体[2E2] to Angiotensin Converting Enzyme 1
  • 特异性Ab11734 recognises human Angiotensin Converting Enzyme.
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, Human, Monkey
  • 免疫原

    Denatured Human kidney Angiotensin Converting Enzyme.

  • 表位This clone recognises denatured ACE, binding to an epitope in the C domain, and has been reported to be active in Western blotting.
  • 阳性对照
    • For immunohistology use kidney tissue (lung in rat studies).
  • 常规说明This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab11734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use neat - 1/20.

ELISA Use at an assay dependent concentration.
WB 1/50 - 1/100. Predicted molecular weight: 170 (somatic) or 90 (germinal) kDa.

靶标

  • 功能Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
  • 组织特异性Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
  • 疾病相关Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
    Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
  • 序列相似性Belongs to the peptidase M2 family.
  • 翻译后修饰Phosphorylated by CK2 on Ser-1299; which allows membrane retention.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACE 1 antibody
    • ACE antibody
    • ACE T antibody
    • ACE_HUMAN antibody
    • ACE1 antibody
    • Angiotensin converting enzyme somatic isoform antibody
    • Angiotensin converting enzyme testis specific isoform antibody
    • Angiotensin I converting enzyme 1 antibody
    • Angiotensin I converting enzyme antibody
    • Angiotensin I converting enzyme peptidyl dipeptidase A 1 antibody
    • angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript antibody
    • Angiotensin-converting enzyme antibody
    • Carboxycathepsin antibody
    • CD 143 antibody
    • CD143 antibody
    • CD143 antigen antibody
    • DCP 1 antibody
    • DCP antibody
    • DCP1 antibody
    • Dipeptidyl carboxypeptidase 1 antibody
    • Dipeptidyl carboxypeptidase I antibody
    • Kininase II antibody
    • MGC26566 antibody
    • MVCD3 antibody
    • Peptidase P antibody
    • Peptidyl dipeptidase A antibody
    • soluble form antibody
    • Testicular ECA antibody
    see all

Anti-Angiotensin Converting Enzyme 1 antibody [2E2] (ab11734)参考文献

This product has been referenced in:
  • Gomes-Santos IL  et al. Effects of exercise training on circulating and skeletal muscle Renin-Angiotensin system in chronic heart failure rats. PLoS One 9:e98012 (2014). WB ; Rat . Read more (PubMed: 24859374) »
  • Frantz ED  et al. Renin-angiotensin system blockers protect pancreatic islets against diet-induced obesity and insulin resistance in mice. PLoS One 8:e67192 (2013). WB . Read more (PubMed: 23894285) »

See all 8 Publications for this product

Product Wall

Thank you for your response and the new enquiry.
This may depend on the actual antibody itself. If the antibody is raised against a full length or a fragment of recombinant protein, then it would be advisable to use rec. protein as positive contro...

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Thank you for your enquiry regarding ab11734.
Angiotensin Converting Enzyme 1 (ACE 1) is expressed with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Tissue lysates from any of these tissues would be a good positive ...

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Thank you for your recent enquires and for your patience. I now have the following information from the originators of these antibodies regarding the total protein concentration. I am sorry we do not have an accurate concentration, but they have be...

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THank you for contacting us. Ab105925 is from ascites and ab11734 is from tissue culture supernatant. Concentrations are not determined for these types of products as they are not relevant unless the antibody is purified, as they may contain proteins o...

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Thank you for your enquiry. This clone recognizes denatured ACE, binding to an epitope in the C domain. The predicted molecular weight: 170 (somatic) or 90 (germinal) kDa. Please contact us again if you have any additional questions.

Thank you for your enquiry. I am sorry to hear that your customer has been having difficulties with this antiserum. I have read your customer's technical questionnaire and I have a few comments. This antibody was characterised by immunohistoche...

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Thank you for your enquiry. Further to discussion with the source of this antiserum. I have evaluated how your customers approach to IHC-P differs to that in publication: Balyasnikova IV et al. Monoclonal antibodies to denatured human ACE (CD 14...

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Thank you for containing me regarding your customer. I am sorry to hear that you have been having difficulties with this antiserum. I would be interested in details of the antigen retrieval steps that has been performed. This step is crucial in pre...

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Thank you for your enquiry. We don't have the ELISA data available for this antibody. This clone has been reported to be suitable for ELISA by the antibody's originator and we suggest referring to the following publication for additional information: ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"