The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa.
Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.
Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney.
Contains 1 fibrinogen C-terminal domain.
Secreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4.
Hepatic fibrinogen/angiopoietin related protein antibody
Hepatic fibrinogen/angiopoietin-related protein antibody
Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein antibody
PPARG angiopoietin related protein antibody
Weakly similar to angiopoietin 1 [H.sapiens] antibody
Western blot - Anti-Angiopoietin-like 4 antibody (ab47046)
All lanes : Anti-Angiopoietin-like 4 antibody (ab47046) at 1 µg/ml
Lane 1 : Recombinant protein 100 ng/Lane, Reducing Lane 2 : Recombinant protein 10 ng/Lane, Reducing Lane 3 : Recombinant protein 1 ng/Lane, Reducing Lane 4 : Recombinant protein 1 ng/Lane, Non-Reducing Lane 5 : Recombinant protein 10 ng/Lane, Non-Reducing Lane 6 : Recombinant protein 100 ng/Lane, Non-Reducing
Secondary All lanes : Anti-Rabbit HRP-Conjugate at 1/2000 dilution
Inoue T et al. Cross-enhancement of ANGPTL4 transcription by HIF1 alpha and PPAR beta/delta is the result of the conformational proximity of two response elements. Genome Biol15:R63 (2014).
Read more (PubMed: 24721177) »