Anti-Angiopoietin 2抗体[MM0020-1F29] (ab56301)

概述

  • 产品名称Anti-Angiopoietin 2抗体[MM0020-1F29]
    参阅全部 Angiopoietin 2 一抗
  • 描述
    小鼠单克隆抗体[MM0020-1F29] to Angiopoietin 2
  • 特异性This antibody recognizes Angiopoietin 2 in western blot without cross reactivity with Angiopoietin 1.
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant purified full length protein (Human).

  • 阳性对照
    • Human endothelial cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明The IgG fraction of the tissue culture supernatant was purified and then lyophilized from 0.2 µm filtered PBS solution.
  • 克隆单克隆
  • 克隆编号MM0020-1F29
  • 同种��IgG2
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab56301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/200.
WB 1/400 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).

靶标

  • 功能Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
  • 序列相似性Contains 1 fibrinogen C-terminal domain.
  • 结构域The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AGPT 2 antibody
    • Agpt2 antibody
    • ANG 2 antibody
    • ANG-2 antibody
    • ANG2 antibody
    • Angiopoietin 2a antibody
    • Angiopoietin 2B antibody
    • Angiopoietin-2 antibody
    • Angiopoietin2 antibody
    • ANGP2_HUMAN antibody
    • ANGPT 2 antibody
    • Angpt2 antibody
    • Tie2 ligand antibody
    see all

Anti-Angiopoietin 2 antibody [MM0020-1F29] 图像

  • ab56301 at 1/200 dilution, staining Angiopoietin 2 in human placental tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

  • Anti-Angiopoietin 2 antibody [MM0020-1F29] (ab56301) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 47 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

Anti-Angiopoietin 2 antibody [MM0020-1F29] (ab56301)参考文献

ab56301 has not yet been referenced specifically in any publications.

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Thank you for your reply.
I have aligned the human angiopoietin 1 and 2 proteins and the overall sequence homology is only 61%.
I would recommend ab64015, since the immunogen does not align well with Ang1 (63% homology)
http://www.abcam....

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