Anti-Angiopoietin 1抗体(ab95230)

概述

  • 产品名称Anti-Angiopoietin 1抗体
    参阅全部 Angiopoietin 1 一抗
  • 描述
    兔多克隆抗体to Angiopoietin 1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Sheep
    预测可用于: Rat, Cow, Dog, Human, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Angiopoietin 1.

    (Peptide available as ab109637.)

  • 阳性对照
    • This antibody gave a positive signal in both E10 and E11 Mouse embryonic brain tissue lysates and MEF1 cell line.

性能

应用

Our Abpromise guarantee covers the use of ab95230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 57 kDa).

靶标

Anti-Angiopoietin 1 antibody 图像

  • Anti-Angiopoietin 1 antibody (ab95230) at 1 µg/ml + E10 Mouse Embryo Brain Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
    The band observed at 53 kDa could potentially be a cleaved form of Angiopoietin 1 due to the presence of a 19 amino acid signal peptide. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • ICC/IF image of ab95230 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab95230, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • All lanes : Anti-Angiopoietin 1 antibody (ab95230) at 1/500 dilution

    Lane 1 : Sheep fetal extra-embryonic membrane purified protein at 50 µg
    Lane 2 : Sheep fetal extra-embryonic membrane purified protein at 30 µg
    Lane 3 : Sheep fetal extra-embryonic membrane purified protein at 20 µg
    Lane 4 : Sheep fetal extra-embryonic membrane purified protein at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG monoclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 57 kDa


    Exposure time : 90 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-Angiopoietin 1 antibody (ab95230)参考文献

This product has been referenced in:
  • Mohammadi E  et al. Endothelial juxtaposition of distinct adult stem cells activates angiogenesis signaling molecules in endothelial cells. Cell Tissue Res 362:597-609 (2015). Read more (PubMed: 26068799) »
  • Duran JM  et al. Bone-derived stem cells repair the heart after myocardial infarction through transdifferentiation and paracrine signaling mechanisms. Circ Res 113:539-52 (2013). ICC/IF, WB ; Mouse . Read more (PubMed: 23801066) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10 %)
Sample Sheep Purified protein (fetal extra-embryonic membrane)
Specification fetal extra-embryonic membrane
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jul 15 2014

Thank you for contacting us.

I have attached the results of the sequence alignment between mouse and human angiopoietin 1.

The link to our IHC-P protocol is as follows:
http://www.abcam.com/index.html?pageconfig=resource&r...

Read More

Thanks again for the additional data. I think there are a few confounding factors here that make the western blot data difficult to interpret confidentally: 1. I agree with you that the intense 40kDa band is likelyb-actin in both blots, but we can not ...

Read More

Thanks for the additional information. A couple more questions... 1. Are one or more of the samples from the blot considered a negative control, in that it is known to not express Angiopoietin 1? 2. After stripping but before reprobing, was it determin...

Read More

Thanks for providing the western blot images as well as the protocol and sample preparation details. I just have a few additional questions: 1. Was any other antibody usedsuccessfully in western blotting with these same samples? 2. How much protein was...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"