Anti-Angiopoietin 1抗体(ab8451)

概述

  • 产品名称Anti-Angiopoietin 1抗体
    参阅全部 Angiopoietin 1 一抗
  • 描述
    兔多克隆抗体to Angiopoietin 1
  • 经测试应用适用于: IHC-P, ICC/IF, WB, ICC, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human, Pig
  • 免疫原

    Synthetic peptide:

    CNQRRNPENGGRRYNRIQHGQ

    , corresponding to N terminal amino acids 21/40 of Mouse Angiopoietin 1. (Peptide available as ab9076).

  • 常规说明


    Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver. To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody (and the ang-2 antibody, ab8452) give a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • 纯度Whole antiserum
  • Primary antibody说明Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver. To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody (and the ang-2 antibody, ab8452) give a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 57 kDa. Using this antiserum with cell supernatants may result in high background due to other components in the serum. This can be alleviated by immunoprecipitating the antibody:antigen complex before detection, or precipitation using soluble Tie2. These methods result in a clean, strong signal. Both Ang-1 and Ang-2 proteins have predicted MW of 57 kDa and appear on blots close to predicted MW. Additional bands may be seen at approx. 75 kDa which represent highly glycosylated forms of the protein.
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
  • 序列相似性Contains 1 fibrinogen C-terminal domain.
  • 翻译后修饰Glycosylated.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AGP 1 antibody
    • AGP1 antibody
    • AGPT antibody
    • ANG 1 antibody
    • ANG antibody
    • ANG-1 antibody
    • ANG1 antibody
    • Angiopoietin-1 antibody
    • Angiopoietin1 antibody
    • ANGP1_HUMAN antibody
    • ANGPT 1 antibody
    • Angpt1 antibody
    • KIAA0003 antibody
    • OTTHUMP00000227592 antibody
    • OTTHUMP00000227599 antibody
    • OTTHUMP00000227600 antibody
    see all

Anti-Angiopoietin 1 antibody 图像

  • This image is courtesy of Marion Scharpfenecker 2002

    Supernatants of mouse-angiopoietin-expressing endothelial cells.

    Lane 1 - wt endothelial cell
    Lane 2 - mouse Ang-1 (clone 1-8) expressing cells
    Lane 3 - mouse Ang-1 (clone 1-15) expressing cells
    Lane 4 - mouse Ang-2 (clone 2-9) expressing cells

    Supernatants of mouse-angiopoietin-expressing endothelial cells.

    Lane 1 - wt endothelial cell
    Lane 2 - mouse Ang-1 (clone 1-8) expressing cells
    Lane 3 - mouse Ang-1 (clone 1-15) expressing cells
    Lane 4 - mouse Ang-2 (clone 2-9) expressing cells

Anti-Angiopoietin 1 antibody (ab8451)参考文献

This product has been referenced in:
  • Stewart N & Chade AR Renoprotective effects of hepatocyte growth factor in the stenotic kidney. Am J Physiol Renal Physiol 304:F625-33 (2013). Read more (PubMed: 23269649) »
  • Mohamad O  et al. Vector-free and transgene-free human iPS cells differentiate into functional neurons and enhance functional recovery after ischemic stroke in mice. PLoS One 8:e64160 (2013). WB ; Human . Read more (PubMed: 23717557) »

See all 17 Publications for this product

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I changed the review to editing status. You can now change the Abreview by correcting the details.
Please also confirm that the secondary antibody was polyclonal or monoclonal?
ab8451 is a rabbit antibody so se...

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Vielen Dank für diese weiteren Informationen.
Es tut mir Leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen, habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456 und so...

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Es tut mir Leid, dass Sie Probleme mit diesen Antikörpern hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456 und sollte allerdings erst am 5 September bei Ihnen ...

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Thank you for contacting us.

I have attached the results of the sequence alignment between mouse and human angiopoietin 1.

The link to our IHC-P protocol is as follows:
http://www.abcam.com/index.html?pageconfig=resource&r...

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Thank you for taking the time to complete our questionnaire and get back to me.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.
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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody and we are happy to help.

I would like to reassure you that this antibody is tested and covered by our ...

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Thank you for getting back to us. Unfortunately, xxx is away from the office today so I will deal with this enquiry.

I think what xxxx was suggesting was to perform a "no primary" control, in order to determine if the secondary anti...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Thanks again for the additional data. I think there are a few confounding factors here that make the western blot data difficult to interpret confidentally: 1. I agree with you that the intense 40kDa band is likelyb-actin in both blots, but we can not ...

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Thanks for the additional information. A couple more questions... 1. Are one or more of the samples from the blot considered a negative control, in that it is known to not express Angiopoietin 1? 2. After stripping but before reprobing, was it determin...

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