Anti-Angiopoietin 1抗体(ab102015)

概述

  • 产品名称Anti-Angiopoietin 1抗体
    参阅全部 Angiopoietin 1 一抗
  • 描述
    兔多克隆抗体to Angiopoietin 1
  • 经测试应用适用于: ELISA, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Human Angiopoietin 1 aa 225-239 (internal sequence).
    Sequence:

    QGLVTRQTYIIQELEKQL RQTYIIQELEKQLNR


    Database link: Q15389

  • 阳性对照
    • ICC/IF: HeLa cell line

性能

应用

Our Abpromise guarantee covers the use of ab102015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 0.1 - 0.5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.

靶标

Anti-Angiopoietin 1 antibody 图像

  • Immunohistochemical staining of human mammary cancer tissue with ab102015.

  • ICC/IF image of ab102015 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab102015, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Angiopoietin 1 antibody (ab102015)参考文献

This product has been referenced in:
  • Ge X  et al. miR-21-5p alleviates leakage of injured brain microvascular endothelial barrier in vitro through suppressing inflammation and apoptosis. Brain Res 1650:31-40 (2016). Read more (PubMed: 27421180) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry regarding ab102015.

We are not aware of any possible cross-reactivity with Ang-2, so it would be very useful for our customers and for us to have this published article as a reference from your customer.

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (human serum, rat heart, brain, liver, muscle, kidn)
Loading amount 30 µg
Specification human serum, rat heart, brain, liver, muscle, kidn
Gel Running Conditions Reduced Denaturing (15% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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提交于 Apr 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"