概述

  • 产品名称
    Anti-Angiomotin like 1抗体
    参阅全部 Angiomotin like 1 一抗
  • 描述
    兔多克隆抗体to Angiomotin like 1
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Guinea pig, Cow, Cat
  • 免疫原

    Synthetic peptide within residues: LTQEDPQMVY QSARQEPQGQ EHQVDNTVME KQVRSTQPQQ NNEELPTYEE , corresponding to N terminal amino acids 144-193 of Human Angiomotin like 1 (NP_570899)

  • 阳性对照
    • 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab84049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.

靶标

图片

  • Anti-Angiomotin like 1 antibody (ab84049) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 106 kDa
    Observed band size : 106 kDa
  • ICC/IF image of ab84049 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84049, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Skouloudaki K & Walz G YAP1 Recruits c-Abl to Protect Angiomotin-Like 1 from Nedd4-Mediated Degradation. PLoS One 7:e35735 (2012). WB . Read more (PubMed: 22558212) »

See 1 Publication for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (ESC)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
ESC
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Feb 28 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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