概述

  • 产品名称Anti-Angiomotin抗体
    参阅全部 Angiomotin 一抗
  • 描述
    兔多克隆抗体to Angiomotin
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Human Angiomotin.

    (Peptide available as ab97422.)

  • 阳性对照
    • This antibody gave a positive signal in Human Placenta Tissue Lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human skeletal muscle.

性能

应用

Our Abpromise guarantee covers the use of ab85143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).

靶标

  • 功能Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.
  • 组织特异性Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.
  • 序列相似性Belongs to the angiomotin family.
  • 结构域The coiled coil domain interacts directly with the BAR domain of ARHGAP17.
    The angiostatin binding domain (871-1005) allows the binding to angiostatin.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cell junction > tight junction. Localized on the cell surface. May act as a transmembrane protein.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AMOT antibody
    • AMOT_HUMAN antibody
    • Angiomotin antibody
    • Angiomotin p130 isoform antibody
    • KIAA1071 antibody
    see all

Anti-Angiomotin antibody 图像

  • Anti-Angiomotin antibody (ab85143) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 118 kDa
    Observed band size : 118 kDa
    Additional bands at : 250 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutes
  • IHC image of Angiomotin staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85143, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Angiomotin antibody (ab85143)参考文献

ab85143 has not yet been referenced specifically in any publications.

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