概述

  • 产品名称Anti-Angiomotin抗体
    参阅全部 Angiomotin 一抗
  • 描述
    兔多克隆抗体to Angiomotin
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 108-157 (TANKQLAEKE YEGSEDTRKT ISQLFAKNKE SQREKEKLEA ELATARSTNE) of human AMOT (NP_573572).

  • 阳性对照
    • 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab83381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:1562500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 72 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.
  • 组织特异性Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.
  • 序列相似性Belongs to the angiomotin family.
  • 结构域The coiled coil domain interacts directly with the BAR domain of ARHGAP17.
    The angiostatin binding domain (871-1005) allows the binding to angiostatin.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cell junction > tight junction. Localized on the cell surface. May act as a transmembrane protein.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AMOT antibody
    • AMOT_HUMAN antibody
    • Angiomotin antibody
    • Angiomotin p130 isoform antibody
    • KIAA1071 antibody
    see all

Anti-Angiomotin antibody 图像

  • Anti-Angiomotin antibody (ab83381) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 72 kDa
    Observed band size : 72 kDa

Anti-Angiomotin antibody (ab83381)参考文献

ab83381 has not yet been referenced specifically in any publications.

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