Anti-Angiomotin抗体(ab117776)

概述

  • 产品名称Anti-Angiomotin抗体
    参阅全部 Angiomotin 一抗
  • 描述
    兔多克隆抗体to Angiomotin
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 1034-1084 of Human Angiomotin (NP_001106962.1).

  • 阳性对照
    • 293T whole cell lysates.

性能

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应用

Our Abpromise guarantee covers the use of ab117776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 118 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 功能Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.
  • 组织特异性Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.
  • 序列相似性Belongs to the angiomotin family.
  • 结构域The coiled coil domain interacts directly with the BAR domain of ARHGAP17.
    The angiostatin binding domain (871-1005) allows the binding to angiostatin.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cell junction > tight junction. Localized on the cell surface. May act as a transmembrane protein.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AMOT antibody
    • AMOT_HUMAN antibody
    • Angiomotin antibody
    • Angiomotin p130 isoform antibody
    • KIAA1071 antibody
    see all

Anti-Angiomotin antibody 图像

  • All lanes : Anti-Angiomotin antibody (ab117776) at 0.04 µg/ml

    Lane 1 : 293T whole cell lysates at 50 µg
    Lane 2 : 293T whole cell lysates at 15 µg
    Lane 3 : HeLa whole cell lysates at 50 µg

    Developed using the ECL technique

    Predicted band size : 118 kDa


    Exposure time : 3 seconds
  • Detection of Angiomotin by Western Blot of Immunprecipitate.
    ab117776 at 1µg/ml staining Angiomotin in 293T whole cell lysate immunoprecipitated using ab117776 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 1 second.

Anti-Angiomotin antibody (ab117776)参考文献

ab117776 has not yet been referenced specifically in any publications.

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