概述

  • 产品名称
    Anti-Angiogenin抗体
    参阅全部 Angiogenin 一抗
  • 描述
    兔多克隆抗体to Angiogenin
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to N terminal amino acids of Human Angiogenin

  • 阳性对照
    • MCF-7, HeLa and SW620 Cell Lysate

性能

应用

Our Abpromise guarantee covers the use of ab125231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能
    May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
  • 组织特异性
    Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.
  • 疾病相关
    Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9) [MIM:611895]. ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy.
  • 序列相似性
    Belongs to the pancreatic ribonuclease family.
  • 发展阶段
    Low level expression in the developing fetus, increased in the neonate, and maximal in the adult.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALS9 antibody
    • AMYOTROPHIC LATERAL SCLEROSIS antibody
    • ANG 1 antibody
    • ANG antibody
    • ANG I antibody
    • ANG1 antibody
    • ANGI antibody
    • ANGI_HUMAN antibody
    • Angiogenin antibody
    • Angiogenin ribonuclease RNase A family, 5 antibody
    • Epididymis luminal protein 168 antibody
    • HEL168 antibody
    • MGC22466 antibody
    • MGC71966 antibody
    • Ribonuclease 5 antibody
    • Ribonuclease RNase A Family 5 antibody
    • RNase 5 antibody
    • RNASE4 antibody
    • RNASE5 antibody
    see all

图片

  • All lanes : Anti-Angiogenin antibody (ab125231) at 1 µg/ml

    Lane 1 : MCF7 lysate
    Lane 2 : HeLa lysate
    Lane 3 : SW620 lysate


    Predicted band size : 17 kDa
  • ab125231 staining human pancreas cancer tissue using Immunohistochemisty-Formalin/PFA-fixed paraffin-embedded sections.

文献

This product has been referenced in:
  • Sayar I  et al. Necl 4 and RNase 5 Are Important Biomarkers for Gastric and Colon Adenocarcinomas. Med Sci Monit 23:2654-2659 (2017). IHC-P ; Human . Read more (PubMed: 28561015) »

See 1 Publication for this product

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - other (HeLA)
Specification
HeLA
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Username

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提交于 May 14 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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