Androstenedione ELISA试剂盒(ab108672)

概述

  • 产品名称Androstenedione ELISA试剂盒
    参阅全部 Androstenedione 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Control Sera 60 <=10%
    批次间
    样品 n Mean SD CV%
    Control Sera 30 <=9.5%
  • 测试
    1 x 96 test
  • 样品类型
    Serum, Plasma
  • 检测类型Competitive
  • 灵敏度
    = 0.01 ng/ml
  • 范围
    0.1 ng/ml - 10 ng/ml
  • 复检

    100.91 %

  • 实验步骤Multiple steps standard assay
  • 产品概述

    Abcam’s Androstenedione in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Androstenedione in serum and plasma.

     

    A 96-well plate has been precoated with anti-Androstenedione IgG. Samples and the Androstenedione-HRP conjugate are added to the wells, where Androstenedione in the sample competes with the added Androstenedione-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Androstenedione in the sample and the intensity is measured at 450 nm.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

  • 存放说明Store at +4°C. Please refer to protocols.
  • 组件 1 x 96 tests
    10X Washing Solution 1 x 50ml
    Androstenedione Control 1 x 1ml
    Androstenedione Standard 0 – 0 ng/mL 1 x 1ml
    Androstenedione Standard 1 – 0.1 ng/mL 1 x 1ml
    Androstenedione Standard 2 – 0.4 ng/mL 1 x 1ml
    Androstenedione Standard 3 – 1.2 ng/mL 1 x 1ml
    Androstenedione Standard 4 – 4.0 ng/mL 1 x 1ml
    Androstenedione Standard 5 – 10.0 ng/mL 1 x 1ml
    Androstenedione-HRP Conjugate 1 x 21ml
    Anti-Androstenedione IgG Coated Microplate (12 x 8 wells) 1 unit
    Cover foils 1 unit
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • 研究领域
  • 别名
    • 4 androstenedione

应用

Our Abpromise guarantee covers the use of ab108672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent dilution.

Androstenedione ELISA Kit 图像

  • Representative Standard Curve using ab108672

实验方案

Androstenedione ELISA Kit (ab108672)参考文献

ab108672 has not yet been referenced specifically in any publications.

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The Androstenedione Human ELISA Kit (ab108672) is provided with androstenedione standard which allows you to perform a standard curve to assess that the kit is working as expected...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"