Anti-Androgen Receptor (phospho S210 + S213)抗体[156C135.2] (ab45089)
Key features and details
- Mouse monoclonal [156C135.2] to Androgen Receptor (phospho S210 + S213)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Androgen Receptor (phospho S210 + S213)抗体[156C135.2]
参阅全部 Androgen Receptor 一抗 -
描述
小鼠单克隆抗体[156C135.2] to Androgen Receptor (phospho S210 + S213) -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Hamster, Cat, Dog, Pig, Chimpanzee, Monkey, Baboon, Cynomolgus monkey, Hedgehog, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Human Androgen Receptor aa 200-300 (phospho S213).
Database link: A39248 -
阳性对照
- IGF1 stimulated LNCaP cells. IHC-P: human normal prostate FFPE tissue sections
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常规说明
In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kDa band was observed. The serine phosphorylation site recognized by ab45089 has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein G purified -
Primary antibody说明
In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kDa band was observed. The serine phosphorylation site recognized by ab45089 has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen. -
克隆
单克隆 -
克隆编号
156C135.2 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45089于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).
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IHC-P | (1) |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa). |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones. -
组织特异性
Isoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107). -
疾病相关
Androgen insensitivity syndrome
Spinal and bulbar muscular atrophy X-linked 1
Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.
Androgen insensitivity, partial -
序列相似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain. -
翻译后修饰
Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation. -
细胞定位
Nucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1. - Information by UniProt
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数据库链接
- Entrez Gene: 747460 Chimpanzee
- Entrez Gene: 102117720 Cynomolgus monkey
- Entrez Gene: 403588 Dog
- Entrez Gene: 100033980 Horse
- Entrez Gene: 367 Human
- Entrez Gene: 397582 Pig
- Entrez Gene: 100009193 Rabbit
- Entrez Gene: 24208 Rat
see all -
形式
There are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45. -
别名
- AIS antibody
- ANDR_HUMAN antibody
- Androgen nuclear receptor variant 2 antibody
see all
图片
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All lanes : Anti-Androgen Receptor (phospho S210 + S213) antibody [156C135.2] (ab45089) at 1 µg/ml
Lane 1 : LNCaP cells with serum starved cells
Lane 2 : LNCaP cells with IGF1 incubated for 4h at 0.1 µg/ml
Lane 3 : LNCaP cells with incubated with 20 ug LY294002 for 30 min then IGF1 incubated for 4h at 0.1 µg/ml
Predicted band size: 99 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
IHC image of Androgen Receptor (phospho S213 + S210) staining in human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45089, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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Datasheet download
文献 (7)
ab45089 被引用在 7 文献中.
- Plyler ZE et al. Non-obstructive vas deferens and epididymis loss in cystic fibrosis rats. Mech Dev 155:15-26 (2019). PubMed: 30391480
- Robitaille CN et al. Antiandrogenic mechanisms of pesticides in human LNCaP prostate and H295R adrenocortical carcinoma cells. Toxicol Sci 143:126-35 (2015). PubMed: 25324206
- Hong H et al. Testosterone regulates cell proliferation in aggressive fibromatosis (desmoid tumour). Br J Cancer 104:1452-8 (2011). PubMed: 21468052
- Agapova OA et al. A neuroactive steroid 5alpha-androstane-3alpha,17beta-diol regulates androgen receptor level in astrocytes. J Neurochem 98:355-63 (2006). PubMed: 16638015
- Kirkegaard T et al. Observer variation in immunohistochemical analysis of protein expression, time for a change? Histopathology 48:787-94 (2006). PubMed: 16722926
- Bhuiyan MM et al. Down-regulation of androgen receptor by 3,3'-diindolylmethane contributes to inhibition of cell proliferation and induction of apoptosis in both hormone-sensitive LNCaP and insensitive C4-2B prostate cancer cells. Cancer Res 66:10064-72 (2006). PubMed: 17047070
- Lin HK et al. Suppression versus induction of androgen receptor functions by the phosphatidylinositol 3-kinase/Akt pathway in prostate cancer LNCaP cells with different passage numbers. J Biol Chem 278:50902-7 (2003). PubMed: 14555644