重组
RabMAb

Anti-Androgen Receptor抗体[ER179(2)] (ab108341)

概述

  • 产品名称
    Anti-Androgen Receptor抗体[ER179(2)]
    参阅全部 Androgen Receptor 一抗
  • 描述
    兔单克隆抗体[ER179(2)] to Androgen Receptor
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, WB, IHC-P, ChIPmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide within Human Androgen Receptor aa 1-100 (N terminal). The exact sequence is proprietary.

  • 阳性对照
    • T47-D and LnCaP cell lysates. Paraffin-embedded human prostatic adenocarcinoma tissue. Paraffin-embedded human prostate tissue. Human testis
  • 常规说明

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab108341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500.

For unpurified use at 1/100 - 1/250.

WB 1/1000 - 1/10000. Predicted molecular weight: 99 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/250 - 1/500. 

ChIP Use at an assay dependent concentration. PubMed: 23817021
  • 应用说明
    Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能
      Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
      Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
    • 组织特异性
      Isoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107).
    • 疾病相关
      Androgen insensitivity syndrome
      Spinal and bulbar muscular atrophy X-linked 1
      Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.
      Androgen insensitivity, partial
    • 序列相似性
      Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • 结构域
      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain.
    • 翻译后修饰
      Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
      Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
      Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
    • 细胞定位
      Nucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1.
    • Information by UniProt
    • 数据库链接
    • 形式
      There are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45.
    • 别名
      • AIS antibody
      • ANDR_HUMAN antibody
      • Androgen nuclear receptor variant 2 antibody
      • Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody
      • Androgen receptor antibody
      • androgen receptor splice variant 4b antibody
      • AR antibody
      • AR8 antibody
      • DHTR antibody
      • Dihydro testosterone receptor antibody
      • Dihydrotestosterone receptor (DHTR) antibody
      • Dihydrotestosterone receptor antibody
      • HUMARA antibody
      • HYSP1 antibody
      • KD antibody
      • Kennedy disease (KD) antibody
      • NR3C4 antibody
      • Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody
      • Nuclear receptor subfamily 3 group C member 4 antibody
      • SBMA antibody
      • SMAX1 antibody
      • Spinal and bulbar muscular atrophy (SBMA) antibody
      • Spinal and bulbar muscular atrophy antibody
      • Testicular Feminization (TFM) antibody
      • TFM antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling Androgen receptor with Purified ab108341 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue sections labeling Androgen receptor with Purified ab108341 at 1:100 dilution. Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
    • Anti-Androgen Receptor antibody [ER179(2)] (ab108341) at 1/5000 dilution (purified) + LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 99 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-Androgen Receptor antibody [ER179(2)] (ab108341) at 1/1000 dilution (unpurified)

      Lane 1 : LnCAP whole cell lysate (positive control)
      Lane 2 : PC3 whole cell lysate (negative control)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 99 kDa
      Observed band size: 120 kDa (why is the actual band size different from the predicted?)



      Lanes 1 - 2: Merged signal (red and green). Green - ab108341 observed at 120 kDa. Red - loading control, ab181602, observed at 37 kDa.

      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab108341 and ab181602 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at a 1:10000 dilution for 1hr at room temperature and then imaged.

    • Unpurified ab108341, at 1/100, staining Androgen Receptor in LnCaP cells by Immunofluorescence.

    • All lanes : Anti-Androgen Receptor antibody [ER179(2)] (ab108341) at 1/1000 dilution (unpurified)

      Lane 1 : T47 D cell lysate
      Lane 2 : LnCaP cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 99 kDa

    • Unpurified ab108341, at 1/250, staining Androgen Receptor in paraffin-embedded Human prostatic adenocarcinoma tissue by Immunohistochemistry.

    • Unpurified ab108341, at 1/250, staining Androgen Receptor in paraffin-embedded Human prostate tissue by Immunohistochemistry.

    • Unpurified ab108341 showing positive staining in Normal testis tissue.

    • Unpurified ab108341 showing positive staining in Prostatic carcinoma T3 tissue.

    • Unpurified  ab108341 showing positive staining in Breast carcinoma tissue.

    • Unpurified ab108341 showing negative staining in Normal liver tissue.

    文献

    This product has been referenced in:
    • Liao Y  et al. Growth arrest and apoptosis induction in androgen receptor-positive human breast cancer cells by inhibition of USP14-mediated androgen receptor deubiquitination. Oncogene 37:1896-1910 (2018). Human . Read more (PubMed: 29353883) »
    • Hieronymus H  et al. Deletion of 3p13-14 locus spanning FOXP1 to SHQ1 cooperates with PTEN loss in prostate oncogenesis. Nat Commun 8:1081 (2017). Read more (PubMed: 29057879) »

    See all 11 Publications for this product

    客户评价及客户问答

    There are currently no Customer reviews or Questions for ab108341.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册