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Synthetic peptide corresponding to Human Androgen Receptor (N terminal). Sequence is propreitary.
Our Abpromise guarantee covers the use of ab74272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration. PubMed: 24466341|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 mins, followed by cooling at RT for 20 mins.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21430025|
ab74272 staining Androgen Receptor in Mouse neuroblastoma cells by Immunocytochemistry/Immunofluorescence. Cells were fixed in formaldehyde and permeabilized in 0.2% Triton X-100 prior to blocking in 3% BSA for 1 hour. The primary antibody was diluted 1/200 and incubated with the sample for 12 hours at 4°C. The secondary antibody was Alexa Fluor® 594-conjugated Goat anti-Rabbit polyclonal, diluted 1/400.
Formalij fixed/paraffin embedded human prostate carcinoma stained with 1/200 ab74272 (30mins, RT).
ab74272 used in ChIP application.Whole cell lysate from human LNCaP cells was used.Cross linking step (X-ChIP) for 10 minutes in 1% formaldehyde.Taqman detection step.Positive control was PSA enhancer.Negative control region, no antibody control, and in the absence of ligand.25 µl undiluted ab74272 per IP was used.
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