Anti-Androgen Receptor抗体- ChIP Grade (ab74272)

概述

  • 产品名称
    Anti-Androgen Receptor抗体- ChIP Grade
    参阅全部 Androgen Receptor 一抗
  • 描述
    兔多克隆抗体to Androgen Receptor - ChIP Grade
  • 经测试应用
    适用于: ChIP, IP, WB, ICC/IF, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Androgen Receptor (N terminal). Sequence is propreitary.

  • 阳性对照
    • IHC-P: Human prostate carcinoma tissue. WB: LnCap cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab74272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 24466341
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. PubMed: 21430025

靶标

  • 功能
    Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
    Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
  • 组织特异性
    Isoform 2 is mainly expressed in heart and skeletal muscle (PubMed:15634333). Isoform 3 is expressed by basal and stromal cells of prostate (at protein level) (PubMed:19244107).
  • 疾病相关
    Androgen insensitivity syndrome
    Spinal and bulbar muscular atrophy X-linked 1
    Defects in AR may play a role in metastatic prostate cancer. The mutated receptor stimulates prostate growth and metastases development despite of androgen ablation. This treatment can reduce primary and metastatic lesions probably by inducing apoptosis of tumor cells when they express the wild-type receptor.
    Androgen insensitivity, partial
  • 序列相似性
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. In the presence of bound steroid the ligand-binding domain interacts with the N-terminal modulating domain, and thereby activates AR transcription factor activity. Agonist binding is required for dimerization and binding to target DNA. The transcription factor activity of the complex formed by ligand-activated AR and DNA is modulated by interactions with coactivator and corepressor proteins. Interaction with RANBP9 is mediated by both the N-terminal domain and the DNA-binding domain. Interaction with EFCAB6/DJBP is mediated by the DNA-binding domain.
  • 翻译后修饰
    Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
    Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
  • 细胞定位
    Nucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1.
  • Information by UniProt
  • 数据库链接
  • 形式
    There are 2 isoforms produced by alternative splicing. Isoform 1 is also known as: AR-B; isoform 2 is known as AR-A or variant AR45.
  • 别名
    • AIS antibody
    • ANDR_HUMAN antibody
    • Androgen nuclear receptor variant 2 antibody
    • Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody
    • Androgen receptor antibody
    • androgen receptor splice variant 4b antibody
    • AR antibody
    • AR8 antibody
    • DHTR antibody
    • Dihydro testosterone receptor antibody
    • Dihydrotestosterone receptor (DHTR) antibody
    • Dihydrotestosterone receptor antibody
    • HUMARA antibody
    • HYSP1 antibody
    • KD antibody
    • Kennedy disease (KD) antibody
    • NR3C4 antibody
    • Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody
    • Nuclear receptor subfamily 3 group C member 4 antibody
    • SBMA antibody
    • SMAX1 antibody
    • Spinal and bulbar muscular atrophy (SBMA) antibody
    • Spinal and bulbar muscular atrophy antibody
    • Testicular Feminization (TFM) antibody
    • TFM antibody
    see all

Anti-Androgen Receptor antibody - ChIP Grade 图像

  • Lane 1 : Anti-Androgen Receptor antibody - ChIP Grade (ab74272) at 1/50 dilution
    Lane 2 : Anti-Androgen Receptor antibody - ChIP Grade (ab74272) at 1/100 dilution

    Lane 1 : LNCaP Whole Cell
    Lane 2 : LNCaP Whole Cell


    Predicted band size : 99 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • ab74272 used in ChIP application.Whole cell lysate from human LNCaP cells was used.Cross linking step (X-ChIP) for 10 minutes in 1% formaldehyde.Taqman detection step.Positive control was PSA enhancer.Negative control region, no antibody control, and in the absence of ligand.25 µl undiluted ab74272 per IP was used.

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  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma labelling Androgen Receptor with ab74272 at a dilution of 1/200 for 30mins at room temperature.

  • ab74272 staining Androgen Receptor in mouse neuroblastoma cells by Immunocytochemistry/Immunofluorescence. Cells were fixed in formaldehyde and permeabilized in 0.2% Triton X-100 prior to blocking in 3% BSA for 1 hour. The primary antibody was diluted 1/200 and incubated with the sample for 12 hours at 4°C. The secondary antibody was Alexa Fluor® 594-conjugated goat anti-rabbit polyclonal, diluted 1/400.

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  • ab74272 staining Androgen Receptor in adult mouse testis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA and blocked with 5% BSA for 45 minutes at 25°C. Samples were incubated with primary antibody (1/400 in 1% BSA) for 16 hours at 4°C. A TRITC-conjugated donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

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Anti-Androgen Receptor antibody - ChIP Grade (ab74272)参考文献

This product has been referenced in:
  • Deng Q  et al. Androgen Receptor Localizes to Plasma Membrane by Binding to Caveolin-1 in Mouse Sertoli Cells. Int J Endocrinol 2017:3985916 (2017). WB, IP, IF ; Mouse . Read more (PubMed: 28642789) »
  • Song W  et al. ERß1 inhibits metastasis of androgen receptor-positive triple-negative breast cancer by suppressing ZEB1. J Exp Clin Cancer Res 36:75 (2017). Read more (PubMed: 28583190) »

See all 28 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Prostate tissue)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Prostate tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Methanol
Username

Dr. Armen Petrosyan

Verified customer

提交于 Sep 26 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (LNCaP - prostate cancer cells)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
20 µg
Specification
LNCaP - prostate cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Jun 20 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat prostate)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Jun 20 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
capybara Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Testis
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Formaldehyde
Username

Raísa Brito

Verified customer

提交于 Dec 09 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP - prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100
Specification
LNCaP - prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Jun 28 2016

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Tissue sections (adult mouse testis)
Specification
adult mouse testis
Permeabilization
No
Fixative
4% PFA
Username

Dr. Qing Wen

Verified customer

提交于 Dec 31 2014

The immunogen sequence is considered confidential by the laboratory that produces it. We only know that it is located within aa 180-320 of accession P10275, http://www.uniprot.org/uniprot/P10275.

ChIP

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Detection step
Semiquantitative PCR
Sample
Mouse Tissue lysate - whole (epididymis)
Specification
epididymis
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Username

Ms. Jaeeun Lee

Verified customer

提交于 May 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (epididymis)
Specification
epididymis
Fixative
Bouin's solution
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 30 2013

The peptide for ab74272 is commercially not available. I am sorry we cannot provide this.
The peptide ab45603 is suitable to be used with ab19066, not with ab74272.

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