Anti-Ancient ubiquitous protein 1抗体(ab101984)

概述

  • 产品名称Anti-Ancient ubiquitous protein 1抗体
    参阅全部 Ancient ubiquitous protein 1 一抗
  • 描述
    兔多克隆抗体to Ancient ubiquitous protein 1
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptice corresponding to a region between residue 426 and 476 of Human Ancient ubiquitous protein 1 (NP_853553.1).

  • 阳性对照
    • Hela and 293T whole cell lysates

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab101984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 53 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome.
  • 组织特异性Detected in blood platelets and leukocytes (at protein level). Ubiquitous. Highly expressed in placenta, liver, kidney, skeletal muscle, heart and brain.
  • 序列相似性Belongs to the AUP1 family.
    Contains 1 CUE domain.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AA589454 antibody
    • Ancient ubiquitous protein 1 antibody
    • AUP 1 antibody
    • aup1 antibody
    • AUP1_HUMAN antibody
    see all

Anti-Ancient ubiquitous protein 1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Ancient ubiquitous protein 1 with ab101984 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-Ancient ubiquitous protein 1 antibody (ab101984) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 53 kDa


    Exposure time : 3 minutes
  • Detection of Ancient ubiquitous protein 1 by Western Blot of Immunprecipitate.
    Lane 1: ab101984, at 1 µg/ml, staining Ancient ubiquitous protein 1 in HeLa whole cell lysate immunoprecipitated using ab101984 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG
    Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-Ancient ubiquitous protein 1 antibody (ab101984)参考文献

ab101984 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab101984.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"