Anti-Ancient ubiquitous蛋白1抗体(ab101984)
Key features and details
- Rabbit polyclonal to Ancient ubiquitous protein 1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Ancient ubiquitous蛋白1抗体
参阅全部 Ancient ubiquitous protein 1 一抗 -
描述
兔多克隆抗体to Ancient ubiquitous蛋白1 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptice corresponding to a region between residue 426 and 476 of Human Ancient ubiquitous protein 1 (NP_853553.1).
-
阳性对照
- Hela and 293T whole cell lysates
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab101984于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 53 kDa.
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
|
IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 53 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. -
组织特异性
Detected in blood platelets and leukocytes (at protein level). Ubiquitous. Highly expressed in placenta, liver, kidney, skeletal muscle, heart and brain. -
序列相似性
Belongs to the AUP1 family.
Contains 1 CUE domain. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 550 Human
- Omim: 602434 Human
- SwissProt: Q9Y679 Human
- Unigene: 411480 Human
-
别名
- AA589454 antibody
- Ancient ubiquitous protein 1 antibody
- AUP 1 antibody
see all
图片
-
All lanes : Anti-Ancient ubiquitous protein 1 antibody (ab101984) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Ancient ubiquitous protein 1 with ab101984 at 1/1000 (1µg/ml). Detection: DAB.
-
Detection of Ancient ubiquitous protein 1 by Western Blot of Immunprecipitate.
Lane 1: ab101984, at 1 µg/ml, staining Ancient ubiquitous protein 1 in HeLa whole cell lysate immunoprecipitated using ab101984 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with an exposure time of 10 seconds.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab101984 尚未被引用在任何文献中。