Anandamide (ethanol solution),endogenous cannabinoid (ab120087)
Key features and details
- Endogenous cannabinoid
- CAS Number: 94421-68-8
- Supplied in ethanol (5 mg/ml)
- Form / State: Liquid
- Source: Synthetic
概述
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产品名称
Anandamide (ethanol solution),endogenous cannabinoid -
描述
Endogenous cannabinoid -
别名
- AEA
- Arachidonylethanolamide
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CAS编号
94421-68-8 -
化学结构
性能
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化学名称
(5Z,8Z,11Z,14Z)-N-(2-Hydroxyethyl)icosa-5,8,11,14-tetraenamide -
分子量
347.54 -
分子式
C22H37NO2 -
PubChem识别号
5281969 -
存放说明
Store at -20°C (desiccating conditions). -
溶解度概述
Supplied in ethanol (5 mg/ml) -
处理
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
O=C(CCC/C=CC/C=CC/C=CC/C=CCCCCC)NCCO -
来源
Synthetic
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研究领域
图片
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2D chemical structure image of ab120087, Anandamide (ethanol solution), endogenous cannabinoid
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ab81298 staining FAK (phospho Y397) in SK-N-SH cells treated with anandamide (ethanol solution) (ab120087), by ICC/IF. Increase in FAK (phospho Y397) expression correlates with increased concentration of anandamide (ethanol solution), as described in literature.
The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120087 (anandamide (ethanol solution)) in ethanol, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab81298 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. Membranes are stained in red with WGA.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (3)
ab120087 被引用在 3 文献中.
- Kim J et al. Docosahexaenoyl ethanolamide improves glucose uptake and alters endocannabinoid system gene expression in proliferating and differentiating C2C12 myoblasts. Front Physiol 5:100 (2014). PubMed: 24711795
- Landucci E et al. CB1 receptors and post-ischemic brain damage: studies on the toxic and neuroprotective effects of cannabinoids in rat organotypic hippocampal slices. Neuropharmacology 60:674-82 (2011). PubMed: 21130785
- Brighton PJ et al. Characterization of the endocannabinoid system, CB(1) receptor signalling and desensitization in human myometrium. Br J Pharmacol 164:1479-94 (2011). PubMed: 21486283