Anti-Amylin抗体(ab55411)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to Amylin
  • 特异性
    ab55411 detects endogenous levels of total Amylin protein.
  • 经测试应用
    适用于: IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from human Amylin.

  • 阳性对照
    • Human brain tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: U87-MG

性能

应用

Our Abpromise guarantee covers the use of ab55411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

靶标

  • 功能
    Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.
  • 序列相似性
    Belongs to the calcitonin family.
  • 结构域
    The mature protein is largely unstructured in the absence of a cognate ligand, and has a strong tendency to form fibrillar aggregates. Homodimerization may be the first step of amyloid formation.
  • 翻译后修饰
    Amyloid fibrils are degraded by IDE.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amylin antibody
    • DAP antibody
    • Diabetes associated peptide antibody
    • Diabetes-associated peptide antibody
    • IAP antibody
    • IAPP antibody
    • IAPP_HUMAN antibody
    • Insulinoma amyloid peptide antibody
    • Islet amyloid polypeptide (diabetes associated peptide; amylin) antibody
    • Islet amyloid polypeptide antibody
    see all

图片

  • ICC/IF image of ab55411 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab55411 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab55411 (1/50 dilution), in the presence or absence of the immunizing peptide.Immunohistochemical analysis of paraffin-embedded human brain tissue using ab55411 (1/50 dilution), in the presence or absence of the immunizing peptide.

文献

ab55411 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your enquiry and your interest in our products.
Concerning ab18018 - there is significant sequence homology between human amylin and that of mouse and rat, but this antibody has only been tested with human samples.
Unfortunately...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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