Anti-Amylin抗体(ab55411)

概述

  • 产品名称Anti-Amylin抗体
    参阅全部 Amylin 一抗
  • 描述
    兔多克隆抗体to Amylin
  • 特异性ab55411 detects endogenous levels of total Amylin protein.
  • 经测试应用适用于: IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from human Amylin.

  • 阳性对照
    • Human brain tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: U87-MG

性能

应用

Our Abpromise guarantee covers the use of ab55411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

靶标

  • 功能Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.
  • 序列相似性Belongs to the calcitonin family.
  • 结构域The mature protein is largely unstructured in the absence of a cognate ligand, and has a strong tendency to form fibrillar aggregates. Homodimerization may be the first step of amyloid formation.
  • 翻译后修饰Amyloid fibrils are degraded by IDE.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amylin antibody
    • DAP antibody
    • Diabetes associated peptide antibody
    • Diabetes-associated peptide antibody
    • IAP antibody
    • IAPP antibody
    • IAPP_HUMAN antibody
    • Insulinoma amyloid peptide antibody
    • Islet amyloid polypeptide (diabetes associated peptide; amylin) antibody
    • Islet amyloid polypeptide antibody
    see all

Anti-Amylin antibody 图像

  • ICC/IF image of ab55411 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab55411 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab55411 (1/50 dilution), in the presence or absence of the immunizing peptide.Immunohistochemical analysis of paraffin-embedded human brain tissue using ab55411 (1/50 dilution), in the presence or absence of the immunizing peptide.

Anti-Amylin antibody (ab55411)参考文献

ab55411 has not yet been referenced specifically in any publications.

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Concerning ab18018 - there is significant sequence homology between human amylin and that of mouse and rat, but this antibody has only been tested with human samples.
Unfortunately...

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