概述

  • 产品名称Amylase Assay试剂盒(Colorimetric)
  • 检测方法Colorimetric
  • 测试
    100 x 1 assay
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
  • 检测类型Quantitative
  • 灵敏度
    > 0.2 mU/well
  • 检测时间
    0h 40m
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: all Mammals
  • 产品概述

    Amylase Assay kit (Colorimetric) (ab102523) detects activity of a-amylase through a two-step reaction. a-Amylase will cleave the substrate ethylidene-pNP-G7 to produce smaller fragments that are eventually modified by a-glucosidase, causing the release of a chromophore that can then be measured at OD = 405 nm. The assay can detect a-amylase content as low as 0.2 mU.


    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Amylases are enzymes that break starch down to sugar molecules. a-amylase is the major form of amylase found in humans and other mammals as well as an enzyme present in seeds, or in fungi (baker's yeast for instance). a-amylase is a calcium metalloenzyme, completely unable to function in the absence of calcium. In human physiology, both the salivary and pancreatic amylases are major digestive enzymes. Increased enzyme levels in humans are associated with salivary trauma; mumps due to inflammation of the salivary glands, pancreatitis and renal failure. A simple, direct and automation-ready procedure for measuring a-amylase activity is, therefore, very desirable.

  • 经测试应用适用于: Functional Studiesmore details
  • 平台Microplate

性能

  • 相关性Amylases are enzymes that break starch down to sugar molecules. Alpha-amylase is the major form of amylase found in humans and other mammals as well as an enzyme present in seeds, or in fungi (baker’s yeast for instance). Alpha-amylase is a calcium metalloenzyme, completely unable to function in the absence of calcium. In human physiology, both the salivary and pancreatic amylases are major digestive enzymes. Increased enzyme levels in humans are associated with salivary trauma; mumps due to inflammation of the salivary glands, pancreatitis and renal failure. A simple, direct and automation-ready procedure for measuring alpha-amylase activity is, therefore, very desirable.

应用

Our Abpromise guarantee covers the use of ab102523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Amylase Assay Kit (Colorimetric) 图像

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Amylase activity measured in biological fluids showing activity (mU) per mL of tested sample. Samples were diluted 2 fold.

  • Amylase activity measured in tissue lysates showing activity (mU) per mg of extracted protein.

    Protein concentration for samples varied from 16 mg/mL to 50 mg/mL. Samples were diluted 2 fold.

  • Sample Test

实验方案

Amylase Assay Kit (Colorimetric) (ab102523)参考文献

This product has been referenced in:
  • O'Harte FP  et al. Dogfish glucagon analogues counter hyperglycaemia and enhance both insulin secretion and action in diet-induced obese diabetic mice. Diabetes Obes Metab 18:1013-24 (2016). Read more (PubMed: 27357054) »
  • Mondragon A  et al. Divergent effects of liraglutide, exendin-4, and sitagliptin on beta-cell mass and indicators of pancreatitis in a mouse model of hyperglycaemia. PLoS One 9:e104873 (2014). Read more (PubMed: 25119717) »

See all 3 Publications for this product

Product Wall



I confirm this kit is suitable for measuring mouse serum amylase.

Thank you for contacting us and for your interest in our products.

I am pleased to confirm that the Amylase Assay Kit (ab102523) is suitable for the use with chicken plasma samples. Please do not hesitate to contact us in case you are experien...

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These 2 kit buffers have different components and pH. They cannot be used interchangeably and it is not recommended to perform the extraction with PBS.


The kit buffers would be ideal to extract your tissue. You can use the amylase assay buffer and the trypsin assay buffer.

Thanks.


We are not aware of any lipase interference in this assay.
The reaction principle is mentioned on our datasheet for this product. The complete organic reaction is proprietary information. The same goes for the positive contro...

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Thank you for contacting us.


The protocol can be found by clicking the following link;

http://www.abcam.com/ps/products/102/ab102523/documents/ab102523%20Amylase%20Assay%20Kit%20(Website).pdf

We do not have received any...

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Thank you for your enquiry regarding ab102523.

I can confirm that this kit can be used for detecting Amylase in different sample types such as Cell culture supernatant, Urine, Serum, Plasma, Tissue Extracts, Cell culture media - as well as Ot...

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Thank you for your telephone enquiry this morning.

I can confirm that this kit has not been specifically tested on rat samples, and so regrettably there is no data to provide. However, theoretically it should work in rat and so we can guaran...

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Thank you for contacting us.

Unfortunately, at this time our testing discount program only applies to primary antibodies, proteins, and ELISA kits. I am sorry I could not be more helpful, please let me know if you need any further assistance.

Thank you for contacting Abcam.

I have contacted the lab about using frozen samples with ab102523. If the samples were frozen appropriately (flash frozen followed by storing at -80C) so as to maintain the enzyme activity, it should be compati...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"