概述

  • 产品名称Anti-AMSH抗体
    参阅全部 AMSH 一抗
  • 描述
    兔多克隆抗体to AMSH
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MSDHGDVSLP PEDRVRALSQ LGSAVEVNED IPPRRYFRSG VEIIRMASIY) of Human AMSH (NP_998787).

  • 阳性对照
    • Jurkat cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab81217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

靶标

  • 功能Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Functions at the endosome and is able to oppose the ubiquitin-dependent sorting of receptors to lysosomes. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the peptidase M67C family.
    Contains 1 MPN (JAB/Mov34) domain.
  • 结构域The JAMM motif is essential for the protease activity.
  • 翻译后修饰Phosphorylated after BMP type I receptor activation.
    Ubiquitinated by SMURF2 in the presence of RNF11.
  • 细胞定位Nucleus. Membrane. Cytoplasm. Early endosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AMSH antibody
    • Associated molecule with the SH3 domain of STAM antibody
    • Endosome associated ubiquitin isopeptidase antibody
    • Endosome-associated ubiquitin isopeptidase antibody
    • MGC126516 antibody
    • MGC126518 antibody
    • MICCAP antibody
    • STABP_HUMAN antibody
    • STAM binding protein antibody
    • STAM-binding protein antibody
    • Stambp antibody
    see all

Anti-AMSH antibody 图像

  • Anti-AMSH antibody (ab81217) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

Anti-AMSH antibody (ab81217)参考文献

ab81217 has not yet been referenced specifically in any publications.

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