重组
RabMAb

Anti-AMPK gamma 1抗体[Y308] (ab32508)

概述

  • 产品名称
    Anti-AMPK gamma 1抗体[Y308]
    参阅全部 AMPK gamma 1 一抗
  • 描述
    兔单克隆抗体[Y308] to AMPK gamma 1
  • 特异性
    ab32508 recognises 5’-AMP-activated protein kinase (AMPK).
  • 经测试应用
    适用于: WB, Flow Cyt, IPmore details
    不适用于: ICC or IHC
  • 种属反应性
    与反应: Mouse, Rat, Human, African green monkey
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)

  • 阳性对照
    • Jurkat cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

相关产品

应用

Our Abpromise guarantee covers the use of ab32508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).Can be blocked with AMPK gamma 1 peptide (ab218345).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration. PubMed: 23612997
  • 应用说明
    Is unsuitable for ICC or IHC.
  • 靶标

    • 功能
      AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
    • 序列相似性
      Belongs to the 5'-AMP-activated protein kinase gamma subunit family.
      Contains 4 CBS domains.
    • 结构域
      The AMPK pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins of AMPK, except the presence of a non-phosphorylatable residue in place of Ser. In the absence of AMP this pseudosubstrate sequence may bind to the active site groove on the alpha subunit (PRKAA1 or PRKAA2), preventing phosphorylation by the upstream activating kinase STK11/LKB1.
      The CBS domains mediate binding to AMP, ADP and ATP. 2 sites bind either AMP or ATP, whereas a third site contains a tightly bound AMP that does not exchange. Under physiological conditions AMPK mainly exists in its inactive form in complex with ATP, which is much more abundant than AMP.
    • 翻译后修饰
      Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 5' AMP activated protein kinase gamma 1 subunit antibody
      • 5' AMP activated protein kinase subunit gamma 1 antibody
      • 5''-AMP-activated protein kinase subunit gamma-1 antibody
      • AAKG1_HUMAN antibody
      • AMP activated protein kinase noncatalytic gamma 1 subunit antibody
      • AMPK gamma 1 chain antibody
      • AMPK gamma1 antibody
      • AMPK subunit gamma-1 antibody
      • AMPKg antibody
      • MGC8666 antibody
      • PRKAG 1 antibody
      • PRKAG1 antibody
      • Protein kinase AMP activated gamma 1 non catalytic subunit antibody
      • protein kinase, AMP-activated, noncatalytic gamma-1 antibody
      see all

    Anti-AMPK gamma 1 antibody [Y308] 图像

    • All lanes : Anti-AMPK gamma 1 antibody [Y308] (ab32508) at 1/1000 dilution

      Lane 1 : HEK293 whole cell lysate
      Lane 2 : HEK293 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      Alexa Fluor® 690-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 38 kDa
      Observed band size : 38 kDa


      Exposure time : 5 minutes

      This image is courtesy of an anonymous Abreview

      See Abreview

    • Anti-AMPK gamma 1 antibody [Y308] (ab32508) at 1/1000 dilution + Jurkat cell lysate

      Predicted band size : 38 kDa
      Observed band size : 38 kDa
    • AMPK gamma 1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10ug of Rabbit monoclonal [Y308] to AMPK gamma 1and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Jurkat whole cell extractdiluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32508. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Bands: 37kDa: AMPK gamma 1.

    Anti-AMPK gamma 1 antibody [Y308] (ab32508)参考文献

    This product has been referenced in:
    • Obba S  et al. The PRKAA1/AMPKa1 pathway triggers autophagy during CSF1-induced human monocyte differentiation and is a potential target in CMML. Autophagy 11:1114-29 (2015). Read more (PubMed: 26029847) »
    • Lee KM  et al. Functional Effects of a Pathogenic Mutation in Cereblon (CRBN) on the Regulation of Protein Synthesis via the AMPK-mTOR Cascade. J Biol Chem 289:23343-52 (2014). WB ; Mouse . Read more (PubMed: 24993823) »

    See all 8 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Bos taurus Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing (4-15% gradient gel)
    Loading amount
    20 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Julie Kim

    Verified customer

    提交于 Apr 17 2017

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    African Green Monkey Cell lysate - whole cell (COS7)
    Specification
    COS7
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 03 2014

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    Human Cell lysate - whole cell (HEK293)
    Specification
    HEK293
    Blocking step
    Odyssey block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 25 2013

    Western blot

    Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (10% tris glycine)
    Sample
    Mouse Cell lysate - other (mouse primary hepatocytes)
    Specification
    mouse primary hepatocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 May 13 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Cell lysate - nuclear (AMPK gama 1 infected cells)
    Loading amount
    20 µg
    Specification
    AMPK gama 1 infected cells
    Gel Running Conditions
    Reduced Denaturing (12% SDS PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 07 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Skeletal muscle)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Mr. Jonas Treebak

    Verified customer

    提交于 Oct 30 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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