The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. Also regulates cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase and hormone-sensitive lipase. This is a regulatory subunit, may be a positive regulator of AMPK activity. It may also serve as an adapter molecule for the catalytic alpha-subunit.
Belongs to the 5'-AMP-activated protein kinase beta subunit family.
Phosphorylated when associated with the catalytic subunit.
ab110141, at 3.75 µg/ml, staining PRKAB2 in Human small intestine (submucosal plexus) tissue by immunohistochemistry. After incubation with ab110141, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
ab110141, at 3.75 µg/ml, staining PRKAB2 in Human liver tissue by immunohistochemistry. After incubation with the ab110141, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.