Anti-AMPK beta 1抗体- Aminoterminal end (ab70922)

概述

  • 产品名称Anti-AMPK beta 1抗体- Aminoterminal end
    参阅全部 AMPK beta 1 一抗
  • 描述
    兔多克隆抗体to AMPK beta 1 - Aminoterminal end
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the N terminal region of human AMPK beta 1.

  • 阳性对照
    • Human hepatocarcinoma tissue, Jurkat cell lysate and mouse spleen tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab70922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
  • 应用说明ELISA: 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/100 - 1/500. Detects a band of approximately 32 kDa plus a range of other bands (predicted molecular weight: 30 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
    • 序列相似性Belongs to the 5'-AMP-activated protein kinase beta subunit family.
    • 结构域The glycogen-binding domain may target AMPK to glycogen so that other factors like glycogen-bound debranching enzyme or protein phosphatases can directly affect AMPK activity.
    • 翻译后修饰Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 1300015D22Rik antibody
      • 5''-AMP-activated protein kinase subunit beta-1 antibody
      • AAKB1_HUMAN antibody
      • AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1 antibody
      • AMP-activated, noncatalytic, beta-1 antibody
      • AMPK antibody
      • AMPK beta 1 chain antibody
      • AMPK subunit beta-1 antibody
      • AMPK-BETA-1 antibody
      • AMPKb antibody
      • AU021155 antibody
      • E430008F22 antibody
      • HAMPKb antibody
      • MGC17785 antibody
      • PRKAB1 antibody
      • Protein kinase AMP activated non catalytic subunit beta 1 antibody
      see all

    Anti-AMPK beta 1 antibody - Aminoterminal end 图像

    • All lanes : Anti-AMPK beta 1 antibody - Aminoterminal end (ab70922) at 1/100 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Mouse spleen tissue lysate

      Lysates/proteins at 12.5 µg per lane.


      Predicted band size : 30 kDa
      Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    • Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue reacted with ab70922 at a 1:50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    Anti-AMPK beta 1 antibody - Aminoterminal end (ab70922)参考文献

    ab70922 has not yet been referenced specifically in any publications.

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