Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)抗体(ab72845)


  • 产品名称Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)抗体
  • 描述
    兔多克隆抗体to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)
  • 特异性Antibody detects endogenous AMPK alpha only when phosphorylated at SDGEFLR(pT)SCGSPNY. The antibody detects both alpha1 and alpha2 isoforms.
  • 经测试应用适用于: WB, IP, ELISAmore details
  • 种属反应性
    与反应: Rat, Chicken, Human
    预测可用于: Mouse, Cow, Dog不与反应: Drosophila melanogaster
  • 免疫原

    Synthetic peptide corresponding to Human AMPK alpha 1 (phospho T183). Also within AMPK alpha 2 (phospho T172). It is identical among Human, mouse, rat, chicken, bovine, dog, and insect species.

  • 阳性对照
    • Serum starved HEK293 whole cell lysate.


Our Abpromise guarantee covers the use of ab72845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.

Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody 图像

  • All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab72845) at 1/500 dilution

    Lane 1 : Molecular weight markers
    Lane 2 : Whole cell lysate from serum starved HEK 293 cells
    Lane 3 : BSA negative control

    Predicted band size : 63 kDa
    Observed band size : 63 kDa

Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab72845)参考文献

This product has been referenced in:
  • Zou D  et al. Protective effects of myricetin on acute hypoxia-induced exercise intolerance and mitochondrial impairments in rats. PLoS One 10:e0124727 (2015). WB ; Rat . Read more (PubMed: 25919288) »
  • Stone JD  et al. AMP-activated protein kinase inhibits vascular smooth muscle cell proliferation and migration and vascular remodeling following injury. Am J Physiol Heart Circ Physiol 304:H369-81 (2013). Rat . Read more (PubMed: 23203966) »

See all 6 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (0-4hr embryos)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 30 µg
Treatment control shRNA and SNF1A shRNA embryos
Specification 0-4hr embryos
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. Richelle Sopko

Verified customer

提交于 Mar 04 2014

free of charge replacement sent.

Thank you for calling Abcam earlier today.
I am sorry about the issues you have been having with ab72845 in western blot. As we discussed I am sending you a new vial of the antibody and you should receive that tomorrow morning. For your reference, ...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Gracias por contactarnos y tomaros la molestia de responder nuestro cuestionario.   He estudiado el protocolo enviado, y hay una serie de sugerencias que me gustaría haceros para intentar mejorar los resultados del anticuerpo. De cualquier manera, al e...

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