RabMAb

Anti-AMPK alpha 1 (phospho S496)抗体[EPR3051] (ab92701)

概述

  • 产品名称Anti-AMPK alpha 1 (phospho S496)抗体[EPR3051]
    参阅全部 AMPK alpha 1 一抗
  • 描述
    兔单克隆抗体[EPR3051] to AMPK alpha 1 (phospho S496)
  • 经测试应用适用于: WB, IP, ICC/IFmore details
    不适用于: Flow Cyt or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    A phospho-specific peptide corresponding to residues surrounding Serine 487 of Human AMPK alpha 1.

  • 阳性对照
    • 293T cell lysate; HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

应用

Our Abpromise guarantee covers the use of ab92701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
ICC/IF
  • 应用说明ICC/IF: 1/100 - 1/250.
    IP: 1/10 - 1/100.
    WB: 1/1000 - 1/10000. Predicted molecular weight: 64 kDa.

    Is unsuitable for Flow Cyt or IHC-P.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
    • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 5 AMP activated protein kinase alpha 1catalytic subunit antibody
      • 5 AMP activated protein kinase catalytic alpha 1 chain antibody
      • 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
      • 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
      • AAPK1 antibody
      • AAPK1_HUMAN antibody
      • ACACA kinase antibody
      • acetyl CoA carboxylase kinase antibody
      • AI194361 antibody
      • AI450832 antibody
      • AL024255 antibody
      • AMP -activate kinase alpha 1 subunit antibody
      • AMP-activated protein kinase, catalytic, alpha -1 antibody
      • AMPK 1 antibody
      • AMPK alpha 1 antibody
      • AMPK alpha 1 chain antibody
      • AMPK antibody
      • AMPK subunit alpha-1 antibody
      • AMPK1 antibody
      • AMPKa1 antibody
      • AMPKalpha1 antibody
      • C130083N04Rik antibody
      • cb116 antibody
      • EC 2.7.11.1 antibody
      • HMG CoA reductase kinase antibody
      • HMGCR kinase antibody
      • hormone sensitive lipase kinase antibody
      • Hydroxymethylglutaryl CoA reductase kinase antibody
      • im:7154392 antibody
      • kinase AMPK alpha1 antibody
      • MGC33776 antibody
      • MGC57364 antibody
      • OTTHUMP00000161795 antibody
      • OTTHUMP00000161796 antibody
      • PRKAA 1 antibody
      • PRKAA1 antibody
      • Protein kinase AMP activated alpha 1 catalytic subunit antibody
      • SNF1-like protein AMPK antibody
      • SNF1A antibody
      • Tau protein kinase PRKAA1 antibody
      • wu:fa94c10 antibody
      see all

    Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] 图像

    • All lanes : Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701) at 1/1000 dilution

      Lane 1 : 293T cell lysate untreated
      Lane 2 : 293T cell lysate treated with LP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 64 kDa
    • Immunofluorescent staining of AMPK alpha 1 in HeLa cells using ab92701 at 1/100 dilution.

    Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] (ab92701)参考文献

    This product has been referenced in:
    • Wang YG & Yang TL Liraglutide reduces fatty degeneration in hepatic cells via the AMPK/SREBP1 pathway. Exp Ther Med 10:1777-1783 (2015). WB ; Human . Read more (PubMed: 26640549) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"