Anti-AMPK alpha 1 (phospho S487)抗体(ab131357)
Key features and details
- Rabbit polyclonal to AMPK alpha 1 (phospho S487)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-AMPK alpha 1 (phospho S487)抗体
参阅全部 AMPK alpha 1 一抗 -
描述
兔多克隆抗体to AMPK alpha 1 (phospho S487) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human AMPK alpha 1 (phospho S487) conjugated to keyhole limpet haemocyanin.
Database link: Q13131 -
阳性对照
- HUVEC cell extract treated with UV, HeLa cells, Human colon carcinoma tissue
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Antibody was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131357于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/500 - 1/1000. Predicted molecular weight: 64 kDa.
|
IHC-P |
1/50 - 1/100.
|
|
ICC/IF |
1/100 - 1/200.
|
说明 |
---|
WB
1/500 - 1/1000. Predicted molecular weight: 64 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
靶标
-
功能
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
-
数据库链接
- Entrez Gene: 5562 Human
- Entrez Gene: 105787 Mouse
- Entrez Gene: 65248 Rat
- Omim: 602739 Human
- SwissProt: Q13131 Human
- SwissProt: Q5EG47 Mouse
- SwissProt: P54645 Rat
- Unigene: 43322 Human
see all -
别名
- 5 AMP activated protein kinase alpha 1catalytic subunit antibody
- 5 AMP activated protein kinase catalytic alpha 1 chain antibody
- 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
see all
图片
-
All lanes : Anti-AMPK alpha 1 (phospho S487) antibody (ab131357) at 1/500 dilution
Lane 1 : HUVEC cell extract untreated
Lane 2 : HUVEC cell extract treated with UV
Predicted band size: 64 kDa -
Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labelling AMPK alpha 1 (phospho S487) with ab131357 antibody at a dilution of 1/50. Picture on the right was preincubated with blocking peptide.
-
Immunofluorescence of methanol-fixed HeLa cells staining AMPK alpha 1 (phospho S487) with ab131357 antibody at a dilution of 1/100.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (23)
ab131357 被引用在 23 文献中.
- Wang PW et al. Irisin alleviates vascular calcification by inhibiting VSMC osteoblastic transformation and mitochondria dysfunction via AMPK/Drp1 signaling pathway in chronic kidney disease. Atherosclerosis 346:36-45 (2022). PubMed: 35255258
- Gao F et al. SESN1 attenuates the Ox‑LDL‑induced inflammation, apoptosis and endothelial‑mesenchymal transition of human umbilical vein endothelial cells by regulating AMPK/SIRT1/LOX1 signaling. Mol Med Rep 25:N/A (2022). PubMed: 35293601
- Cai C et al. Empagliflozin attenuates cardiac microvascular ischemia/reperfusion through activating the AMPKα1/ULK1/FUNDC1/mitophagy pathway. Redox Biol 52:102288 (2022). PubMed: 35325804
- Xiao N et al. Metformin abrogates pathological TNF-α-producing B cells through mTOR-dependent metabolic reprogramming in polycystic ovary syndrome. Elife 11:N/A (2022). PubMed: 35748536
- Luo P et al. S-Allylmercaptocysteine improves alcoholic liver disease partly through a direct modulation of insulin receptor signaling. Acta Pharm Sin B 11:668-679 (2021). PubMed: 33777674