The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: use at 15 to 25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain.