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Anti-AMPK alpha 1抗体(ab58166)

概述

  • 产品名称Anti-AMPK alpha 1抗体
    参阅全部 AMPK alpha 1 一抗
  • 描述
    小鼠单克隆抗体to AMPK alpha 1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: LQLYQVDSRT YLLDFRSIDD EITEAKSGTA TPQRSGSVSN YRSCQRSDSD AEAQGKSSEV SLTSSVTSLD SSPVDLTPRP GSHTIEFFEM CANLIKILAQ , corresponding to amino acids 451-551 of Human AMPK alpha 1

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应用

Our Abpromise guarantee covers the use of ab58166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 63 kDa.

靶标

  • 功能Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5 AMP activated protein kinase alpha 1catalytic subunit antibody
    • 5 AMP activated protein kinase catalytic alpha 1 chain antibody
    • 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
    • 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
    • AAPK1 antibody
    • AAPK1_HUMAN antibody
    • ACACA kinase antibody
    • acetyl CoA carboxylase kinase antibody
    • AI194361 antibody
    • AI450832 antibody
    • AL024255 antibody
    • AMP -activate kinase alpha 1 subunit antibody
    • AMP-activated protein kinase, catalytic, alpha -1 antibody
    • AMPK 1 antibody
    • AMPK alpha 1 antibody
    • AMPK alpha 1 chain antibody
    • AMPK antibody
    • AMPK subunit alpha-1 antibody
    • AMPK1 antibody
    • AMPKa1 antibody
    • AMPKalpha1 antibody
    • C130083N04Rik antibody
    • cb116 antibody
    • EC 2.7.11.1 antibody
    • HMG CoA reductase kinase antibody
    • HMGCR kinase antibody
    • hormone sensitive lipase kinase antibody
    • Hydroxymethylglutaryl CoA reductase kinase antibody
    • im:7154392 antibody
    • kinase AMPK alpha1 antibody
    • MGC33776 antibody
    • MGC57364 antibody
    • OTTHUMP00000161795 antibody
    • OTTHUMP00000161796 antibody
    • PRKAA 1 antibody
    • PRKAA1 antibody
    • Protein kinase AMP activated alpha 1 catalytic subunit antibody
    • SNF1-like protein AMPK antibody
    • SNF1A antibody
    • Tau protein kinase PRKAA1 antibody
    • wu:fa94c10 antibody
    see all

Anti-AMPK alpha 1 antibody 图像



  • Predicted band size : 63 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: AMPK knockout HAP1 cell lysate (20 µg)

    Lane 3: MCF7 cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab58166 observed at 63 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab58166 was shown to recognize AMPK when AMPK knockout samples were used, along with additional cross-reactive bands. Wild-type and AMPK knockout samples were subjected to SDS-PAGE. ab58166 and ab181602 (loading control to GAPDH) were diluted 1 µg/mL and 1/2000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.



  • Predicted band size : 63 kDa
    AMPK alpha 1 antibody (ab58166) at 1ug/lane + HepG2 cell lysate at 25ug/lane.

Anti-AMPK alpha 1 antibody (ab58166)参考文献

ab58166 has not yet been referenced specifically in any publications.

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