Anti-Amphiregulin抗体- BSA and Azide free (ab31344)

概述

  • 产品名称
    Anti-Amphiregulin抗体- BSA and Azide free
    参阅全部 Amphiregulin 一抗
  • 描述
    兔多克隆抗体to Amphiregulin - BSA and Azide free
  • 特异性
    Highly specific to amphiregulin. No cross-reactivity with EGF, TGFa, or HB-EGF.
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    RKKKGGKNGKNRRNRKKKN

    , corresponding to amino acids 26-44 of secreted Human Amphiregulin

  • 阳性对照
    • LS174T; phorbol ester treated MCF-7 breast cancer cells; epithelial cells of mucosal surface in normal colon.
  • 常规说明


    Directed against the region of amphiregulin which binds to heparin.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein A purified
  • Primary antibody说明
    Directed against the region of amphiregulin which binds to heparin.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab31344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 28 kDa.
IP Use at an assay dependent dilution.

靶标

文献

This product has been referenced in:
  • Puttabyatappa M  et al. EGF-like ligands mediate progesterone's anti-apoptotic action on macaque granulosa cells. Biol Reprod 88:18 (2013). Rhesus monkey . Read more (PubMed: 23136296) »

See 1 Publication for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mammary gland)
Specification
Mammary gland
Fixative
Acetone:methanol (1:1)
Permeabilization
Yes - 0.025% Triton X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Verified customer

提交于 Dec 02 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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