Anti-Amphiphysin抗体[C14-23] (ab16770)

概述

  • 产品名称Anti-Amphiphysin抗体[C14-23]
    参阅全部 Amphiphysin 一抗
  • 描述
    小鼠单克隆抗体[C14-23] to Amphiphysin
  • 经测试应用适用于: ICC/IF, Flow Cyt, ELISA, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fusion protein, corresponding to amino acids 320-696 of Human Amphiphysin.

  • 阳性对照
    • Mouse brain extract.

性能

应用

Our Abpromise guarantee covers the use of ab16770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 76 kDa.
IP Use a concentration of 1 - 2 µg/ml.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
  • 组织特异性Neurons, certain endocrine cell types and spermatocytes.
  • 序列相似性Contains 1 BAR domain.
    Contains 1 SH3 domain.
  • 细胞定位Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amp antibody
    • AMPH 1 antibody
    • AMPH antibody
    • AMPH_HUMAN antibody
    • AMPH1 antibody
    • Amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen) antibody
    • Amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen) antibody
    • Amphiphysin antibody
    • Amphiphysin I antibody
    • CG8604 antibody
    • Damp antibody
    • dAmph antibody
    • Dmel\CG8604 antibody
    • HGNC:471 antibody
    • MGC49429 antibody
    • Stiff man syndrome with breast cancer 128kD autoantigen antibody
    • Stiff man syndrome with breast cancer 128kDa autoantigen antibody
    see all

Anti-Amphiphysin antibody [C14-23] 图像



  • Predicted band size : 76 kDa


    ab16770 at 1/2000 dilution staining Amphiphysin in mouse and rat brain by Western blot. Lane 1: Mouse brain. Lane 2: Rat brain. Top band: Amphiphysin 1 Lower band: Amphiphysin 2
  • ICC/IF image of ab16770 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16770, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Overlay histogram showing SH-SY5Y cells stained with ab16770 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16770, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Amphiphysin antibody [C14-23] (ab16770)参考文献

ab16770 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab16770.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"