概述

  • 产品名称
    Ammonia Assay试剂盒
    参阅全部 Ammonia 试剂盒
  • 样品类型
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • 检测类型
    Quantitative
  • 灵敏度
    20 µM
  • 检测时间
    1h 00m
  • 产品概述

    Ammonia Assay Kit (ab83360) provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of ammonia and ammonium. In the assay, ammonia and ammonium are converted to a product that reacts with the OxiRed probe to generate color (λmax = 570 nm) which can be easily quantified by plate reader. The kit can detect 1 nmol (~20 µM) of total ammonia and ammonium, which is much more sensitive than measuring ammonia with a NADPH based assay.


    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology such as acid/base balance.

  • 经测试应用
    适用于: Functional Studiesmore details

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 标识符 100 tests
    Ammonia Assay Buffer WM 1 x 25ml
    Converting Enzyme (Lyophilized) Blue 1 vial
    Developer Orange 1 vial
    Enzyme Mix (Lyophilized) Green 1 vial
    NH4Cl Standard Yellow 1 x 100µl
    OxiRed Probe in DMSO Red 1 x 200µl
  • 研究领域
  • 相关性
    Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology like normal animal acid/base balance.

应用

Our Abpromise guarantee covers the use of ab83360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

图片

  • Ammonia measured in cell culture medium (range of dilution 1:10-1:200) and control medium (range of dilution 1:1-1:20), background signal subtracted (duplicates +/- SD).

  • Ammonia measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).

  • Ammonia measured in cell lysates, background signal subtracted (duplicates +/- SD).

  • Ammonia measured in biological fluids (range of dilution 1:1-1:100), background signal subtracted (duplicates +/- SD).

  • Example of ammonia standard curve using ab83360

实验方案

文献

This product has been referenced in:

See all 5 Publications for this product

客户评价及客户问答

The kit detects Ammonia which would exist as ammonium ion in solution. The detection principle is based on reaction with ammonium ion in solution. This ammonium ion population includes free pre-existing cellular ammonium and the ionized form of ammonia...

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In response to your questions on this kit, the pH of the sample should be ˜7-8. To use mouse stool sample, add assay buffer to sample. Mix it completely either by pipetting up & down, sonicating or vortexing. Centr...

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This kit measures the total ammonia + ammonium concentration in your samples. Ammonium does not interfere with this assay.

The actual method and way the kit does this is proprietary information and so I am unable to provide more information. ...

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Phenol red containing media can interfere with the results of this assay. Diluting the media samples with assay buffer may help reduce the concentration of the phenol red thus minimizing the background.

My advice is to switch to medium that ...

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For this kit, we prefer measuring from fresh samples for best results but cell culture media can be centrifuged to remove any debris and then stored at -80C for future assay.

For serum or plasma samples, there are no special requirements for preparation of serum or plasma samples for the ammonia assay.

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This kit has been tested on mouse brain, heart, liver kidney and muscle tissues. Please see figure 3 in the protocol booklet for more information.
Please do not hesitate to contact us if you need any more adv...

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Yes, storage at -80 C until you are ready to perform the assay will be fine.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. ...

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By following the tissue preparation protocol you should be able to attain as much sample material as needed to perform numerous tests with this assay. Adding 100ul of Assay buffer to your samples and using a d...

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1)The pH of the assay buffer is 8.0.
2) It is not necessary to deproteinate samples with this kit.

Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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