概述

  • 产品名称Ammonia Assay试剂盒
    参阅全部 Ammonia 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • 检测类型Quantitative
  • 灵敏度
    20 µM
  • 检测时间
    1h 00m
  • 产品概述

    Ammonia Assay Kit (ab83360) provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of Ammonia or Ammonium. In the assay, Ammonia or Ammonium is converted to a product that reacts with the OxiRed probe to generate color (?max = 570 nm) which can be easily quantified by plate reader. The kit can detect 1 nmol (~20 µM) of ammonia or ammonium, much more sensitive than measuring NADPH based ammonia assay.

    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology such as acid/base balance.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology like normal animal acid/base balance.

应用

Our Abpromise guarantee covers the use of ab83360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Ammonia Assay Kit 图像

  • Ammonia measured in cell culture medium and control medium, background signal subtracted (duplicates +/- SD).

  • Ammonia measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).

  • Ammonia measured in cell lysates, background signal subtracted (duplicates +/- SD).

  • Ammonia measured in biological fluids, background signal subtracted (duplicates +/- SD).

  • Example of ammonia standard curve using ab83360

实验方案

Ammonia Assay Kit (ab83360)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Thank you for contacting Abcam earlier today.

This kit detects ammonia, but not ammonium. I know that you emailed a week ago, asking a similar question regarding the chemical reaction table for this kit. I am still waiting to hear back from ...

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COMPATIBILITY OF THE KIT WITH ORGANIC SOLVENTS

Excellent Good 4/5 (Ease of Use)
Abreviews
The ammonia determination is accurate for samples of tissue extracted in methanol:acetonitrile:water (5:3:2) solution, provided that the samples are diluted 1:5 in assay buffer. The reaction efficiency decreases when the ratio between organic solvents and assay buffer increases. The reaction does not occur if the samples in organic solvents are not further diluted in assay buffer.
Figure Legend. The standard curve has been generated by mixing in each well 10 microliters of methanol, acetonitrile, water, solution containing known amounts of ammonia, to 40 microliters of assay buffer. The reaction started with the addition of 50microliters/well of reaction mix. Samples were incubated at 37 degrees for 1 hour.
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Abcam user community

Verified customer

提交于 Jul 27 2016

For this kit, we prefer measuring from fresh samples for best results but cell culture media can be centrifuged to remove any debris and then stored at -80C for future assay.

Abreviews
We had good results using the ABCAM ammonia kit for the detection of NH3 in our cell lysates. However, we wanted to see the distribution of NH3 in the intact cells to determine the intercellular variation. We were able to adapt the kit to allow for the in situ detection of ammonia. We chose two cell lines one strongly + for NH3 and one -. The key was to use formalin fixed cells AND to digest in protease prior to use. Under these conditions, we got an excellent signal in the + cells and no signal in the - cells.
Username

Abcam user community

Verified customer

提交于 Dec 27 2013

The kit detects Ammonia which would exist as ammonium ion in solution. The detection principle is based on reaction with ammonium ion in solution. This ammonium ion population includes free pre-existing cellular ammonium and the ionized form of ammonia...

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We have not received any complaints from the other customers who bought this same kit and same lot number. Its possible there was a problem with reconstitution and storage of the kit components.


Important points to consider regarding th...

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For serum or plasma samples, there are no special requirements for preparation of serum or plasma samples for the ammonia assay. For the beta HB assay, it is recommended (see section 4 of the protocol) that samples be de-proteinated with a 10 kDa filte...

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Thank you for contacting us.
In response to your questions on this kit, the pH of the sample should be ˜7-8. To use mouse stool sample, add assay buffer to sample. Mix it completely either by pipetting up & down, sonicating or vortexing. Centr...

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Thanks for your enquiry and for providing the standard curve data.
The standard curve data you provided look good and are quite similar to the example standard curve seen on the online product datasheet for this product. If you could, please furth...

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Thank you for your patience.

I have checked with the lab about more details about the kit's mechanism. Thefollowingdetails are the ones they can give out:

Our assay buffer contains some ingredients which can interact with the ammon...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"