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Ammonia Assay Kit (ab83360) provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of ammonia and ammonium. In the assay, ammonia and ammonium are converted to a product that reacts with the OxiRed probe to generate color (λmax = 570 nm) which can be easily quantified by plate reader. The kit can detect 1 nmol (~20 µM) of total ammonia and ammonium, which is much more sensitive than measuring ammonia with a NADPH based assay.
Visit our FAQs page for tips and troubleshooting.
Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology such as acid/base balance.
|Ammonia Assay Buffer||WM||1 x 25ml|
|Converting Enzyme (Lyophilized)||Blue||1 vial|
|Enzyme Mix (lyophilized)||Green||1 vial|
|NH4Cl Standard||Yellow||1 x 100µl|
|OxiRed Probe in DMSO||Red||1 x 200µl|
Our Abpromise guarantee covers the use of ab83360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Ammonia measured in cell culture medium (range of dilution 1:10-1:200) and control medium (range of dilution 1:1-1:20), background signal subtracted (duplicates +/- SD).
Ammonia measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).
Ammonia measured in cell lysates, background signal subtracted (duplicates +/- SD).
Ammonia measured in biological fluids (range of dilution 1:1-1:100), background signal subtracted (duplicates +/- SD).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"