Anti-Aminopeptidase A抗体[1C4] (ab119798)

概述

  • 产品名称Anti-Aminopeptidase A抗体[1C4]
    参阅全部 Aminopeptidase A 一抗
  • 描述
    小鼠单克隆抗体[1C4] to Aminopeptidase A
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein corresponding to Human Aminopeptidase A. Produced in HEK293T cells.
    Database link: NP_001968

  • 阳性对照
    • HEK293T cells transfected with Aminopeptidase A, Human Kidney, Carcinoma of Human kidney, Human liver and Human endometrium tissues, COS7 cells transiently transfected with Aminopeptidase A, Hela cells
  • 常规说明Dilute in PBS (pH7.3) before use.

性能

应用

Our Abpromise guarantee covers the use of ab119798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Predicted molecular weight: 109 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

靶标

  • 功能Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.
  • 组织特异性Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.
  • 序列相似性Belongs to the peptidase M1 family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Aminopeptidase A antibody
    • AMPE_HUMAN antibody
    • AP-A antibody
    • APA antibody
    • bp-1 antibody
    • bp1 antibody
    • CD249 antibody
    • Differentiation antigen gp160 antibody
    • EAP antibody
    • Enpep antibody
    • Glutamyl aminopeptidase (aminopeptidase A) antibody
    • Glutamyl aminopeptidase antibody
    • Gp160 antibody
    • Ly51 antibody
    see all

Anti-Aminopeptidase A antibody [1C4] 图像

  • All lanes : Anti-Aminopeptidase A antibody [1C4] (ab119798) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY BP1

    Lysates/proteins at 5 µg/ml per lane.


    Predicted band size : 109 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC210521)
  • Immunohistochemical staining of paraffin-embedded Human Kidney tissue using ab119798 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human Kidney tissue using ab119798 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Human liver tissue using ab119798 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Human endometrium tissue using ab119798 at a dilution of 1/150.
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BP1 using ab119798 at a dilution of 1/100.
  • HEK293T cells transfected with either RC210521 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab119798 at a dilution of 1/100, and then analyzed by flow cytometry.
  • Flow cytometric Analysis of Hela cells, using ab119798 at a dilution of 1/100 (Red), compared to a nonspecific negative control antibody (Blue).

Anti-Aminopeptidase A antibody [1C4] (ab119798)参考文献

ab119798 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Permeabilization No
Sample Human Cell (hESC H9)
Specification hESC H9
Gating Strategy Doublet discrimination gating/live-dead discrimination (PI)/GFP-expressing cells
Preparation Cell harvesting/tissue preparation method: mild trypsinisation/non-enzymatic dissociation
Sample buffer: FACS staining buffer
Username

Dr. Dmitry Shaposhnikov

Verified customer

提交于 Dec 18 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"