Anti-Amino-terminal enhancer of split抗体(ab16638)

概述

  • 产品名称
    Anti-Amino-terminal enhancer of split抗体
    参阅全部 Amino-terminal enhancer of split 一抗
  • 描述
    兔多克隆抗体to Amino-terminal enhancer of split
  • 经测试应用
    适用于: IP, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human GRG (Groucho homologue).

    (Peptide available as ab16684.)

  • 阳性对照
    • This antibody gives a positive signal in WB against Recombinant His-tagged GRG

性能

应用

Our Abpromise guarantee covers the use of ab16638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 22 kDa).

靶标

  • 功能
    Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.
  • 组织特异性
    Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
  • 序列相似性
    Belongs to the WD repeat Groucho/TLE family.
  • 结构域
    Lacks the C-terminal WD repeats.
  • 翻译后修饰
    Ubiquitinated by XIAP/BIRC4.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Aes 1 antibody
    • Aes 2 antibody
    • aes antibody
    • AES_HUMAN antibody
    • Aes1 antibody
    • Aes2 antibody
    • Amino enhancer of split antibody
    • Amino terminal enhancer of split antibody
    • Amino-terminal enhancer of split antibody
    • Esp 1 antibody
    • Esp1 antibody
    • Gp130 associated protein GAM antibody
    • Gp130-associated protein GAM antibody
    • GRG 5 antibody
    • GRG antibody
    • GRG protein antibody
    • Grg-5 antibody
    • GRG5 antibody
    • Groucho-related protein 5 antibody
    • Protein ESP 1 antibody
    • Protein ESP1 antibody
    • Protein GRG antibody
    • TLE 5 antibody
    • TLE5 antibody
    see all

图片

  • All lanes : Anti-Amino-terminal enhancer of split antibody (ab16638) at 1 µg/ml

    Lane 1 : As above
    Lane 2 : As above
    Lane 3 : As above
    Lane 4 : As above
    Lane 5 : As above
    Lane 6 : Human Amino-terminal enhancer of split peptide (ab16684) at 1 µg/ml
    Lane 7 : Human Amino-terminal enhancer of split peptide (ab16684) at 1 µg/ml
    Lane 8 : Human Amino-terminal enhancer of split peptide (ab16684) at 1 µg/ml
    Lane 9 : Human Amino-terminal enhancer of split peptide (ab16684) at 1 µg/ml
    Lane 10 : Human Amino-terminal enhancer of split peptide (ab16684) at 1 µg/ml


    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa (possible cross reactivity),28 kDa (possible cross reactivity),33 kDa (possible cross reactivity),40 kDa (possible cross reactivity),53 kDa (possible cross reactivity),75 kDa (possible cross reactivity),80 kDa (possible cross reactivity).

文献

ab16638 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (ES cells)
Specification
ES cells
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS/Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jan 27 2012

Thank you very much for your interest in ab86016, ab16638 and ab62555. To our knowledge, these antibodies have not been tested in with IF. ab16638, although predicted to react with mouse GRG, this has also not been tested as yet. Therefore, I c...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (HeLa)
Total protein in input
3e+007 cells
Specification
HeLa
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

提交于 Apr 20 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (HeLa, HepG2, K562, GM12878, Jurkat)
Loading amount
10 µg
Specification
HeLa, HepG2, K562, GM12878, Jurkat
Gel Running Conditions
Reduced Denaturing (10-14% Tris-HCl)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 14 2010

Thank you for your patience. I have received confirmation that the sequence from this antibody cross reacts with Q08117 (197AA long) and Q14CJ1 (264AA long). There is no indication of this cross reacting with any other forms though on looking at Q08...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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