Anti-gp78抗体(ab101284)
Key features and details
- Rabbit polyclonal to gp78
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-gp78抗体
参阅全部 gp78 一抗 -
描述
兔多克隆抗体to gp78 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Orangutan -
免疫原
Synthetic peptide corresponding to Human gp78 aa 500-550.
Database link: NP_001135.3 -
阳性对照
- HeLa and 293T whole cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab101284于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 73 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
E3 ubiquitin-protein ligase which can target both itself and other proteins including CD3D and APOB for proteasomal degradation. Mediates polyubiquitination of CYP3A4. Specific receptor for the autocrine motility factor. Mediates tumor invasion and metastasis. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Promotes ubiquitination of misfolded proteins such as mutant CFTR; proposed to mediate sequential ubiquitination by recognizing already ubiquitin-conjugated substrates and to cooperate with E3 ubiquitin-protein ligase RNF5. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 CUE domain.
Contains 1 RING-type zinc finger. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 735643 Chimpanzee
- Entrez Gene: 267 Human
- Omim: 603243 Human
- SwissProt: Q9UKV5 Human
- Unigene: 295137 Human
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别名
- AMF receptor antibody
- AMF receptor isoform 1 antibody
- AMF receptor isoform 2 antibody
see all
图片
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All lanes : Anti-gp78 antibody (ab101284) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 73 kDa
Exposure time: 3 minutes -
Detection of Human gp78 in immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab101284 at 3 µg/mg lysate for immunoprecipitation and at 1 µg/ml for subsequent Western blotting.
Detection by chemiluminescence with an exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab101284 被引用在 1 文献中.
- Ramachandran S et al. SYVN1, NEDD8, and FBXO2 Proteins Regulate ?F508 Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Ubiquitin-mediated Proteasomal Degradation. J Biol Chem 291:25489-25504 (2016). PubMed: 27756846