概述

  • 产品名称Anti-Amelotin抗体
    参阅全部 Amelotin 一抗
  • 描述
    兔多克隆抗体to Amelotin
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen, corresponding to amino acids 16-110 (SLPQLKPALG LPPTKLAPDQ GTLPNQQQSN QVFPSLSLIP LTQMLTLGPD LHLLNPAAGM TPGTQTHPLT LGGLNVQQQL HPHVLPIFVT QLGAQ) of Human Amelotin.

  • 阳性对照
    • Human cerebellum tissue.

性能

应用

Our Abpromise guarantee covers the use of ab122312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-Amelotin antibody 图像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122312 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122312, at 1/150, staining Amelotin in paraffin embedded Human cerebellum tissue by Immunohistochemistry.

Anti-Amelotin antibody (ab122312)参考文献

This product has been referenced in:
  • Lee HS  et al. Comparative gene-expression analysis of the dental follicle and periodontal ligament in humans. PLoS One 8:e84201 (2013). IHC-P ; Human . Read more (PubMed: 24376796) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (follicle)
Specification follicle
Permeabilization No
Fixative Paraformaldehyde
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提交于 Apr 02 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (tooth)
Specification tooth
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"